About: Application of flow cytometry to Saccharomyces cerevisiae population analysis

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Tato práce byla zaměřena na optimalizaci a aplikaci vhodných metod fluorescenčního značení k charakterizaci buněk Saccharomyces cerevisiae. Využita byla tři různá fluorescenční barviva, propidium jodid, k determinaci buněčné viability a obsahu DNA, fluorescein diacetát k měření okamžité enzymové aktivity a fluorescein isothiokyanát ke sledování obsahu intracelulárních bílkovin. (cs) This study was focused on a development and an application of a rapid and reliable staining method for characterisation of Saccharomyces cerevisiae cells. Three different fluorescent probes such as propidium iodide (PI), fluorescein diacetate (FDA) and fluorescein isothiocyanate (FITC) were used for staining. PI was used to determine cell viability in a native sample and DNA content in sample fixed by ethanol. To assess protein distribution in yeast population the FITC amine-reactive probe was used. Instantaneous cell enzyme activity was measured as an amount of fluorescein liberated from FDA by intracellular esterase activity. This study was focused on a development and an application of a rapid and reliable staining method for characterisation of Saccharomyces cerevisiae cells. Three different fluorescent probes such as propidium iodide (PI), fluorescein diacetate (FDA) and fluorescein isothiocyanate (FITC) were used for staining. PI was used to determine cell viability in a native sample and DNA content in sample fixed by ethanol. To assess protein distribution in yeast population the FITC amine-reactive probe was used. Instantaneous cell enzyme activity was measured as an amount of fluorescein liberated from FDA by intracellular esterase activity. (en)
Title	Application of flow cytometry to Saccharomyces cerevisiae population analysis Aplikace průtokové cytometrie v analýze kvasinek Saccharomyces cerevisiae (cs) Application of flow cytometry to Saccharomyces cerevisiae population analysis (en)
skos:prefLabel	Application of flow cytometry to Saccharomyces cerevisiae population analysis Aplikace průtokové cytometrie v analýze kvasinek Saccharomyces cerevisiae (cs) Application of flow cytometry to Saccharomyces cerevisiae population analysis (en)
skos:notation	RIV/60461373:22330/05:00019125!RIV08-MSM-22330___
http://linked.open.../vavai/riv/strany	745-748
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(1P05OE189), P(GA525/03/0375)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Melzoch, Karel Rychtera, Mojmír Branská, Barbora
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	512810
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/05:00019125
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Saccharomyces cerevisiae; flow cytometry; fluorescence microscopy; viability; nucleic acid content; protein content; esterase activity (en)
http://linked.open.../riv/klicoveSlovo	viability esterase activity flow cytometry Saccharomyces cerevisiae protein content nucleic acid content fluorescence microscopy
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[16B0B2F4B20F]
http://linked.open...i/riv/nazevZdroje	Chimia
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Large-scale implementation of an optimum technology for high-productivity processes with recombinant methylotrophic yeasts Image analyses of microbial population dynamics
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	59
http://linked.open...iv/tvurceVysledku	Melzoch, Karel Paulová, Leona Rychtera, Mojmír Sekavová, Barbora
issn	0009-4293
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	22330
is http://linked.open...avai/riv/vysledek of	Application of flow cytometry to Saccharomyces cerevisiae population analysis

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