About: Determination of egg white lysozyme by on-line coupled CITP-CZE technique

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Chemical Technology Prague, Prague, Czech Republic An on-line coupled capillary isotachophoresis # capillary zone electrophoresis method for the determination of lysozyme in selected food products is described. The optimized electrolyte system consisted of 10 mM NH4OH 20 mM acetic acid (leading electrolyte), 5 mM -aminocaproic acid 5 mM acetic acid (terminating electrolyte), and 20 mM -aminocaproic acid 5mM acetic acid 0.1% m/v hydroxypropylmethylcellulose (background electrolyte). A clear separation of lysozyme from other components of acidic sample extract was achieved within 15 min. Method characteristics, i.e., linearity (0#50 g/mL), accuracy (recovery 96 5%), intra-assay (3.8%), quantification limit (1 g/ml), and detection limit (0.25 g/mL) were determined. Low laboriousness, sufficient sensitivity and low running costs are important attributes of this method. The developed method is suitable for the quantification of the egg content in egg pasta. Chemical Technology Prague, Prague, Czech Republic An on-line coupled capillary isotachophoresis # capillary zone electrophoresis method for the determination of lysozyme in selected food products is described. The optimized electrolyte system consisted of 10 mM NH4OH 20 mM acetic acid (leading electrolyte), 5 mM -aminocaproic acid 5 mM acetic acid (terminating electrolyte), and 20 mM -aminocaproic acid 5mM acetic acid 0.1% m/v hydroxypropylmethylcellulose (background electrolyte). A clear separation of lysozyme from other components of acidic sample extract was achieved within 15 min. Method characteristics, i.e., linearity (0#50 g/mL), accuracy (recovery 96 5%), intra-assay (3.8%), quantification limit (1 g/ml), and detection limit (0.25 g/mL) were determined. Low laboriousness, sufficient sensitivity and low running costs are important attributes of this method. The developed method is suitable for the quantification of the egg content in egg pasta. (en)
Title	Determination of egg white lysozyme by on-line coupled CITP-CZE technique Determination of egg white lysozyme by on-line coupled CITP-CZE technique (en)
skos:prefLabel	Determination of egg white lysozyme by on-line coupled CITP-CZE technique Determination of egg white lysozyme by on-line coupled CITP-CZE technique (en)
skos:notation	RIV/60461373:22330/03:00008446!RIV/2004/MSM/223304/N
http://linked.open.../vavai/riv/strany	860-864
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 223300004), Z(MSM 223300005)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Kvasnička, František
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoká škola chemicko-technologická v Praze / Fakulta potravinářské a biochemické technologie
http://linked.open...dnocenehoVysledku	603349
http://linked.open...ai/riv/idVysledku	RIV/60461373:22330/03:00008446
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Capillary isotachophoresis / Capillary zone electrophoresis/ Egg white / Lysozyme (en)
http://linked.open.../riv/klicoveSlovo	Capillary isotachophoresis / Capillary zone electrophoresis/ Egg white / Lysozyme
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[ECB080AABE22]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	24
http://linked.open...iv/tvurceVysledku	Kvasnička, František
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20223300004 http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20223300005
issn	0173-0835
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	22330

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