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  • Six water-soluble macrocyclic photosensitizers, the members of two groups of expanded porphyrins (metallotexaphyrins and free-base sapphyrins) containing hydrophilic substituents and meso-tetra(4-sulfonatophenyl)-porphyrin, were tested by UV-Vis absorption and resonance Raman spectroscopy in the in vitro nibding experiments with intact mitochondria isolated form swine liver. Studied macrocycles showed markedly differenrt affinity to mitochondria. The highest uptake was observed for sapphyrin-sugar conjugate and metal-lotexaphirins. Sapphirin-polyamine conjugates exhibit something less affinity to mitochondria, while the porphyrin of anionic character showed very low mitochondrial uptake. Obtained spectroscopic results confirm that the binding process altered the self-aggregatio degree fo expanded porphyrins.
  • Six water-soluble macrocyclic photosensitizers, the members of two groups of expanded porphyrins (metallotexaphyrins and free-base sapphyrins) containing hydrophilic substituents and meso-tetra(4-sulfonatophenyl)-porphyrin, were tested by UV-Vis absorption and resonance Raman spectroscopy in the in vitro nibding experiments with intact mitochondria isolated form swine liver. Studied macrocycles showed markedly differenrt affinity to mitochondria. The highest uptake was observed for sapphyrin-sugar conjugate and metal-lotexaphirins. Sapphirin-polyamine conjugates exhibit something less affinity to mitochondria, while the porphyrin of anionic character showed very low mitochondrial uptake. Obtained spectroscopic results confirm that the binding process altered the self-aggregatio degree fo expanded porphyrins. (en)
Title
  • In vitro interaction of macrocyclic photosensitizers with intact mitochondria: a spectroscopic study
  • In vitro interaction of macrocyclic photosensitizers with intact mitochondria: a spectroscopic study (en)
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  • In vitro interaction of macrocyclic photosensitizers with intact mitochondria: a spectroscopic study
  • In vitro interaction of macrocyclic photosensitizers with intact mitochondria: a spectroscopic study (en)
skos:notation
  • RIV/60461373:22330/03:00007579!RIV/2004/MSM/223304/N
http://linked.open.../vavai/riv/strany
  • 85-96
http://linked.open...avai/riv/aktivita
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  • Z(MSM 223400008)
http://linked.open...iv/cisloPeriodika
  • 1
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  • 610061
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  • RIV/60461373:22330/03:00007579
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  • Macrocyclic photosensitizer; In vitro mitochondrial uptake; UV-VIS absorption spectroscopy; Resonance Raman spectroscopy (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [0610E212EC2B]
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  • Biochimica and Biophysica Acta (BBA) - general subjects
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http://linked.open...v/svazekPeriodika
  • 1620
http://linked.open...iv/tvurceVysledku
  • Král, Vladimír
  • Volka, Karel
  • Sessler, J. L.
  • Sedláčková, Vladimíra
  • Sinica, Alla
  • Sinica, Andrij
http://linked.open...n/vavai/riv/zamer
issn
  • 0006-3002
number of pages
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  • 22330
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