About: Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method

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About: Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method Goto Sponge Distinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The broth microdilution (BMD) method is widely used for the determination of minimum inhibitory concentrations of antimicrobial agents, including volatile oils and their components. In this series of various experiments, we have demonstrated the influence of thymoquinone (TQ) vapor on the results of the BMD test performed with Staphylococcus aureus as a model organism. The spread of vapor from the TQ containing wells (32–512 µg/mL) caused the complete inhibition of staphylococcal growth in adjoining wells initially containing bacterium-inoculated pure Mueller–Hinton broth only and thus produced false positive results of the test. The ability of TQ to pass into the adjoined wells was subsequently confirmed by gas chromatography–mass spectrometry, whereas TQ at concentrations up to 84 µg/mL was detected in these wells after five hours. Based on these results, we suppose that vapors of TQ as well as of other naturally occurring volatile compounds and their mixtures (for example essential oils and plant The broth microdilution (BMD) method is widely used for the determination of minimum inhibitory concentrations of antimicrobial agents, including volatile oils and their components. In this series of various experiments, we have demonstrated the influence of thymoquinone (TQ) vapor on the results of the BMD test performed with Staphylococcus aureus as a model organism. The spread of vapor from the TQ containing wells (32–512 µg/mL) caused the complete inhibition of staphylococcal growth in adjoining wells initially containing bacterium-inoculated pure Mueller–Hinton broth only and thus produced false positive results of the test. The ability of TQ to pass into the adjoined wells was subsequently confirmed by gas chromatography–mass spectrometry, whereas TQ at concentrations up to 84 µg/mL was detected in these wells after five hours. Based on these results, we suppose that vapors of TQ as well as of other naturally occurring volatile compounds and their mixtures (for example essential oils and plant (en)
Title	Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method (en)
skos:prefLabel	Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method Thymoquinone vapor significantly affects the results of Staphylococcus aureus sensitivity tests using the standard broth microdilution method (en)
skos:notation	RIV/60460709:41340/14:63809!RIV15-MSM-41340___
http://linked.open...avai/riv/aktivita	O Z
http://linked.open...avai/riv/aktivity	O, Z(MSM6046070901)
http://linked.open...iv/cisloPeriodika	N
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Rondevaldová, Johana Kokoška, Ladislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Česká zemědělská univerzita v Praze / Fakulta tropického zemědělství
http://linked.open...dnocenehoVysledku	50502
http://linked.open...ai/riv/idVysledku	RIV/60460709:41340/14:63809
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	essential oil, microtiter plate, Nigella sativa, quinone, Staphylococcus aureus, volatile (en)
http://linked.open.../riv/klicoveSlovo	Staphylococcus aureus essential oil microtiter plate quinone Nigella sativa volatile
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[70E00D544F3C]
http://linked.open...i/riv/nazevZdroje	Fitoterapia
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	94
http://linked.open...iv/tvurceVysledku	Kokoška, Ladislav Kouřimská, Lenka Havlík, Jaroslav Klouček, Pavel Nový, Pavel Rondevaldová, Johana
http://linked.open...ain/vavai/riv/wos	000335608700013
http://linked.open...n/vavai/riv/zamer	Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje.
issn	0367-326X
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	41340

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