About: Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture

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About: Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture Goto Sponge Distinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2–3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50–60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013. In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2–3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50–60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013. (en)
Title	Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture (en)
skos:prefLabel	Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture Blueberry red ringspot virus Eliminated from Highbush Blueberry by Shoot Tip Culture (en)
skos:notation	RIV/60077344:_____/14:00433365!RIV15-AV0-60077344
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Přibylová, Jaroslava Špak, Josef Pavingerová, Daniela Špaková, Vlastimila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	5593
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/14:00433365
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	BRRV; in vitro; Vaccinium corymbosum L. (en)
http://linked.open.../riv/klicoveSlovo	in vitro BRRV Vaccinium corymbosum L.
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[F1D06029FE64]
http://linked.open...i/riv/nazevZdroje	Plant Protection Science
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	50
http://linked.open...iv/tvurceVysledku	Pavingerová, Daniela Přibylová, Jaroslava Špak, Josef Špaková, Vlastimila Sedlák, J. Paprštein, F.
issn	1212-2580
number of pages	5 (xsd:int)

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