About: Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

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About: Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis Goto Sponge Distinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR. Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR. (en)
Title	Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis (en)
skos:prefLabel	Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis (en)
skos:notation	RIV/60077344:_____/13:00396848!RIV14-AV0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P V
http://linked.open...avai/riv/aktivity	I, P(GAP502/11/1471), P(QH71290), V
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Vlasák, Josef Bříza, Jindřich Niedermeierová, Hana Pavingerová, Daniela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	92061
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/13:00396848
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cry3A gene modification; Picea abies; Agrobacterium tumefaciens (en)
http://linked.open.../riv/klicoveSlovo	Agrobacterium tumefaciens Picea abies Cry3A gene modification
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[A7F5481456C6]
http://linked.open...i/riv/nazevZdroje	Acta Biochimica Polonica
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of transgenic tissue lines of spruce (Picea abies) showing high toxicity towards bark beatle (Scolytidae) species. Study of mechanisms of insect pests resistance against Bacillus thuringiensis Cry toxins
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Pavingerová, Daniela Vlasák, Josef Bříza, Jindřich Niedermeierová, Hana
http://linked.open...ain/vavai/riv/wos	000325237000017
issn	0001-527X
number of pages	6 (xsd:int)

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