About: Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Transformation of plants is a popular tool for modifying various desirable traits. Marker genes, like those encoding for bacterial beta-glucuronidase (GUS), firefly luciferase (LUC) or jellyfish green fluorescent protein (GFP) have been shown to be very useful for establishing of efficient transformation protocols. Due to favourable properties such as no need of exogenous substrates and easy visualization, GFP has been found to be superior in to other markers in many cases. However, the use of GFP fluorescence is associated with some obstacles, mostly related to the diminishing of green fluorescence in older tissues, variation in fluorescence levels among different tissues and organs, and occasional interference with other fluorescing compounds in plants. This paper briefly summarizes basic GFP properties and applications, and describes in more detail the contribution of GFP to the establishment, evaluation and improvement of transformation procedures for plants. Moreover, features and possible obstac Transformation of plants is a popular tool for modifying various desirable traits. Marker genes, like those encoding for bacterial beta-glucuronidase (GUS), firefly luciferase (LUC) or jellyfish green fluorescent protein (GFP) have been shown to be very useful for establishing of efficient transformation protocols. Due to favourable properties such as no need of exogenous substrates and easy visualization, GFP has been found to be superior in to other markers in many cases. However, the use of GFP fluorescence is associated with some obstacles, mostly related to the diminishing of green fluorescence in older tissues, variation in fluorescence levels among different tissues and organs, and occasional interference with other fluorescing compounds in plants. This paper briefly summarizes basic GFP properties and applications, and describes in more detail the contribution of GFP to the establishment, evaluation and improvement of transformation procedures for plants. Moreover, features and possible obstac (en)
Title	Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants (en)
skos:prefLabel	Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants (en)
skos:notation	RIV/60076658:12220/06:00010933!RIV10-MSM-12220___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6007665806)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hraška, Marek Rakouský, Slavomír Čurn, Vladislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Jihočeská univerzita v Českých Budějovicích / Zemědělská fakulta
http://linked.open...dnocenehoVysledku	477303
http://linked.open...ai/riv/idVysledku	RIV/60076658:12220/06:00010933
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bombardment; plant transformation; selection (en)
http://linked.open.../riv/klicoveSlovo	bombardment selection plant transformation
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[2BA50E8D6D75]
http://linked.open...i/riv/nazevZdroje	Plant Cell Tissue Organ Culture
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	86
http://linked.open...iv/tvurceVysledku	Hraška, Marek Rakouský, Slavomír Čurn, Vladislav
http://linked.open...n/vavai/riv/zamer	Sustainable methods of agricultural management in montane and submontane areas with respect to harmonization of the production and nonproduction spheres
issn	0167-6857
number of pages	16 (xsd:int)
http://localhost/t...ganizacniJednotka	12220
is http://linked.open...avai/riv/vysledek of	Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants

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