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| rdf:type
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| rdfs:seeAlso
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| Description
| - Chlorinated ethenes (CE) are the second most common soil contaminant worldwide; hence there is long-term experience with a range of clean-up techniques for such pollutants. One of the most successful methods is enhanced reductive dehalogenation (ERD), which utilises anaerobic microbial degradation of CE, stimulated by organic substrate application in situ. At some sites, however, cis1,2-DCE and cancerous vinyl chloride have been shown to accumulate due to a reduction in ERD efficiency, though the mechanisms are not fully understood. A deeper insight into the key-factors affecting the ERD process, therefore, is highly desirable. Here, we demonstrate our initial test of nanofibre biomass carrier composed of sample stabilisation for transport to the laboratory, isolation DNA and PCR amplification . We aim to accelerate, automate and increase the precision of pre-treatment biomass sampling for DNA isolation by replacing expensive and time consuming groundwater filtration by newly developed trap-samplers that include nanofibre-carriers. Nanofibre carriers have a high specific surface, resulting in higher biomass grow, and hence DNA yield. The DNA yield was very comparable for all stabilization procedures. All DNA samples were able to be amplified by PCR and qPCR methods without any presence of inhibition. Nanofibre biomass carriers are still under testing procedure, we aim to compare the microbial community in the groundwater, soil and the carrier, but the first results are very promising.
- Chlorinated ethenes (CE) are the second most common soil contaminant worldwide; hence there is long-term experience with a range of clean-up techniques for such pollutants. One of the most successful methods is enhanced reductive dehalogenation (ERD), which utilises anaerobic microbial degradation of CE, stimulated by organic substrate application in situ. At some sites, however, cis1,2-DCE and cancerous vinyl chloride have been shown to accumulate due to a reduction in ERD efficiency, though the mechanisms are not fully understood. A deeper insight into the key-factors affecting the ERD process, therefore, is highly desirable. Here, we demonstrate our initial test of nanofibre biomass carrier composed of sample stabilisation for transport to the laboratory, isolation DNA and PCR amplification . We aim to accelerate, automate and increase the precision of pre-treatment biomass sampling for DNA isolation by replacing expensive and time consuming groundwater filtration by newly developed trap-samplers that include nanofibre-carriers. Nanofibre carriers have a high specific surface, resulting in higher biomass grow, and hence DNA yield. The DNA yield was very comparable for all stabilization procedures. All DNA samples were able to be amplified by PCR and qPCR methods without any presence of inhibition. Nanofibre biomass carriers are still under testing procedure, we aim to compare the microbial community in the groundwater, soil and the carrier, but the first results are very promising. (en)
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| Title
| - Nanofibre Biomass Carriers as a Valuable Tool for Analysis of Microbial Community At Polluted Locality
- Nanofibre Biomass Carriers as a Valuable Tool for Analysis of Microbial Community At Polluted Locality (en)
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| skos:prefLabel
| - Nanofibre Biomass Carriers as a Valuable Tool for Analysis of Microbial Community At Polluted Locality
- Nanofibre Biomass Carriers as a Valuable Tool for Analysis of Microbial Community At Polluted Locality (en)
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| skos:notation
| - RIV/46747885:24620/14:#0000619!RIV15-TA0-24620___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/46747885:24620/14:#0000619
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - nanofibre carriers; molecular genetic analysis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/nazevZdroje
| - Biotech 2014 & 6th Czech - Swiss Symposium with Exhibition
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Lederer, Tomáš
- Ševců, Alena
- Czinnerová, Marie
- Dolinová, Iva
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
| - Vysoká škola chemicko-technologická v Praze
|
| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
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