About: PCR identification of toxigenic Bacillus cereus isolates from cow´s milk

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Incidence of DNA sequences related to toxin genes were studied in 60 isolates of the B. cereus from cow's milk samples using multiplex PCR. All the isolates harboured genes coded for Nhe and EntFM. The hbl genes were detected in 95 % of the isolates. The isolates in our study in the most cases lack the hblA gene. On the other hand all the isolates carried genes nheA, nheB, nheC and entFM. The frequency of the bceT gene was 30.2% in the isolates. Besides, 60.3% of isolates carried the cytK gene (32% cytK1, 28.3% cytK2). Only 1.6 % of the analysed B. cereus isolates was the emetic type. Among all the tested isolates in this study, only one was emetic type, which suggested that emetic type of B. cereus is not predominant in raw cow's milk. Different toxigenic patterns of the analysed isolates indicated that the B. cereus population in cow's milk is diverse. In: Spore forming bacteria in food, Le Berre Media, Quimper, 2009. ISBN 978-2-9514743-2-1. Incidence of DNA sequences related to toxin genes were studied in 60 isolates of the B. cereus from cow's milk samples using multiplex PCR. All the isolates harboured genes coded for Nhe and EntFM. The hbl genes were detected in 95 % of the isolates. The isolates in our study in the most cases lack the hblA gene. On the other hand all the isolates carried genes nheA, nheB, nheC and entFM. The frequency of the bceT gene was 30.2% in the isolates. Besides, 60.3% of isolates carried the cytK gene (32% cytK1, 28.3% cytK2). Only 1.6 % of the analysed B. cereus isolates was the emetic type. Among all the tested isolates in this study, only one was emetic type, which suggested that emetic type of B. cereus is not predominant in raw cow's milk. Different toxigenic patterns of the analysed isolates indicated that the B. cereus population in cow's milk is diverse. In: Spore forming bacteria in food, Le Berre Media, Quimper, 2009. ISBN 978-2-9514743-2-1. (en)
Title	PCR identification of toxigenic Bacillus cereus isolates from cow´s milk PCR identification of toxigenic Bacillus cereus isolates from cow´s milk (en)
skos:prefLabel	PCR identification of toxigenic Bacillus cereus isolates from cow´s milk PCR identification of toxigenic Bacillus cereus isolates from cow´s milk (en)
skos:notation	RIV/26788462:_____/09:#0000216!RIV10-MSM-26788462
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QF3162), Z(MSM2678846201)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Banykó, Juraj
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Agrovýzkum Rapotín s.r.o.
http://linked.open...dnocenehoVysledku	332910
http://linked.open...ai/riv/idVysledku	RIV/26788462:_____/09:#0000216
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bacillus cereus, toxins, PCR identification, cow milk (en)
http://linked.open.../riv/klicoveSlovo	toxins PCR identification cow milk Bacillus cereus
http://linked.open...ontrolniKodProRIV	[37DEC674B0B0]
http://linked.open...v/mistoKonaniAkce	Quimper
http://linked.open...i/riv/mistoVydani	Quimper
http://linked.open...i/riv/nazevZdroje	Spore forming bacteria in food
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Research of frequency of pathogenic and technological unfavourable microflora in milk, and the development of methods to the increasing of technological and hygienic guarantee of raw milk using to the produce of cheeses.
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Vyletělová, Marcela Banykó, Juraj
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2009-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Uplatnění evropského modelu multifunkčního zemědělství v LFA oblastech České republiky
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Le Berre Media
https://schema.org/isbn	978-2-9514743-2-1
is http://linked.open...avai/riv/vysledek of	PCR identification of toxigenic Bacillus cereus isolates from cow´s milk PCR identification of toxigenic Bacillus cereus isolates from cow´s milk PCR identification of toxigenic Bacillus cereus isolates from cow´s milk PCR identification of toxigenic Bacillus cereus isolates from cow´s milk

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