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rdf:type
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Description
| - Cílem této práce byla izolace a purifikace hlavního imunodominantního proteinu, Outer surface proteinu C (OspC) ze skupiny Borrelia burgdorferi, původce Lymeského onemocnění. Cílem bylo získat protein v množství a čistotě vhodné pro imunizaci experimentálních zvířat. Pro optimalizaci výnosu byla použita iontová afinitní chromatografie za různých podmínek. Nejlepší účinnost byla dosažena za použití HiTrap Chelating Column. (cs)
- The aim of this work was isolation and purification of the major immunodominant protein, Outer surface protein C (OspC) of three members of the species group Borrelia burgdorferi, the causative agent of Lyme disease. The aim was to obtain this protein in a quantity and purity sufficient for immunization of experimental animals. For optimalization of protein purification´s yield was used immobilized metal ion affinity chromatography under different conditions. The greatest efficiency was achieved by using of HiTrap Chelating Column under native conditions.
- The aim of this work was isolation and purification of the major immunodominant protein, Outer surface protein C (OspC) of three members of the species group Borrelia burgdorferi, the causative agent of Lyme disease. The aim was to obtain this protein in a quantity and purity sufficient for immunization of experimental animals. For optimalization of protein purification´s yield was used immobilized metal ion affinity chromatography under different conditions. The greatest efficiency was achieved by using of HiTrap Chelating Column under native conditions. (en)
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Title
| - Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato.
- Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato. (en)
- Izolace a purifikace rekombinantního outer surface proteinu C (rOspC) Borrelia burgdorferi sensu lato. (cs)
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skos:prefLabel
| - Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato.
- Isolation and purification of recombinant outer surface protein C (rOspC) of Borrelia burgdorferi sensu lato. (en)
- Izolace a purifikace rekombinantního outer surface proteinu C (rOspC) Borrelia burgdorferi sensu lato. (cs)
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skos:notation
| - RIV/25304046:_____/05:#0000008!RIV06-MPO-25304046
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/25304046:_____/05:#0000008
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - OspC; Borrelia burgdorferi; recombinant protein; immobilized metal ion affinity chromatography (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Raška, Milan
- Weigl, Evžen
- Křupka, Michal
- Vrzal, Vladimír
- Běláková, Jana
- Tuháčková, Jana
- Šebestová, Martina
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issn
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number of pages
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