About: Results of in vitro chemotherapy of apple cv. Fragrance

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. The results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial field-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with well-developed leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the first chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. The success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity. The effect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. The results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial field-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with well-developed leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the first chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. The success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity. (en)
Title	Results of in vitro chemotherapy of apple cv. Fragrance Results of in vitro chemotherapy of apple cv. Fragrance (en)
skos:prefLabel	Results of in vitro chemotherapy of apple cv. Fragrance Results of in vitro chemotherapy of apple cv. Fragrance (en)
skos:notation	RIV/25271121:_____/13:#0000474!RIV14-MZE-25271121
http://linked.open...avai/predkladatel	Výzkumný a šlechtitelský ústav ovocnářský Holovousy s.r.o.
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH91153), P(QJ1210175)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Sedlák, Jiří Paprštein, František Svobodová, Ladislava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o.
http://linked.open...dnocenehoVysledku	102701
http://linked.open...ai/riv/idVysledku	RIV/25271121:_____/13:#0000474
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ribavirin; PCR; ELISA; virus detection; virus elimination; virus infection (en)
http://linked.open.../riv/klicoveSlovo	virus detection ELISA PCR virus infection virus elimination ribavirin
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[1997195BEF9F]
http://linked.open...i/riv/nazevZdroje	Horticultural Science
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Exploitation of in vitro cultures for recovering of cultivars of fruit trees and grapevines from viruses, phytoplasmas and quarantine pathogens for the system of certification of planting material including verification of quality. Research and development of standard methodic procedures of recovering of fruit trees and grapevinesby chemotherapy of in vitro cultures for certification system of healthy status of planting material.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	40
http://linked.open...iv/tvurceVysledku	Polák, Jaroslav Paprštein, František Sedlák, Jiří Svobodová, Ladislava Gadiou, S.
http://linked.open...ain/vavai/riv/wos	000329605000007
issn	0862-867X
number of pages	5 (xsd:int)

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