About: Maintenance of Vaccinium gene resources in actively growing in vitro cultures

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The possibility of establishment of in vitro duplicate collections of selected highbush blueberry and lingonberry cultivars was investigated. Genotypes of highbush blueberry (Blueray, Bluecrop and Berkeley) and one genotype of lingonberry (Linnea) were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson’s medium (AN) and McCown medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg/l were tested for repeated subcultures. Based on the obtained results, WPM proved to be more suitable for cultivation of selected cultivars in in vitro cultures. Within the same range of zeatin concentration, the four genotypes gave higher multiplication rates on WPM medium. Across all experiments, the highest multiplication rate (4.8) was obtained for ´Berkeley´ on WPM medium with the highest concentration 2 mg/l of zeatin. After three months of storage under normal growing conditions, the survival rate was very high (99–100%). The possibility of establishment of in vitro duplicate collections of selected highbush blueberry and lingonberry cultivars was investigated. Genotypes of highbush blueberry (Blueray, Bluecrop and Berkeley) and one genotype of lingonberry (Linnea) were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson’s medium (AN) and McCown medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg/l were tested for repeated subcultures. Based on the obtained results, WPM proved to be more suitable for cultivation of selected cultivars in in vitro cultures. Within the same range of zeatin concentration, the four genotypes gave higher multiplication rates on WPM medium. Across all experiments, the highest multiplication rate (4.8) was obtained for ´Berkeley´ on WPM medium with the highest concentration 2 mg/l of zeatin. After three months of storage under normal growing conditions, the survival rate was very high (99–100%). (en)
Title	Maintenance of Vaccinium gene resources in actively growing in vitro cultures Maintenance of Vaccinium gene resources in actively growing in vitro cultures (en)
skos:prefLabel	Maintenance of Vaccinium gene resources in actively growing in vitro cultures Maintenance of Vaccinium gene resources in actively growing in vitro cultures (en)
skos:notation	RIV/25271121:_____/09:0R000907!RIV10-MZE-25271121
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH82232)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Sedlák, Jiří Paprštein, František
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	VÝZKUMNÝ A ŠLECHTITELSKÝ ÚSTAV OVOCNÁŘSKÝ HOLOVOUSY s.r.o.
http://linked.open...dnocenehoVysledku	324424
http://linked.open...ai/riv/idVysledku	RIV/25271121:_____/09:0R000907
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	highbush blueberry; lingonberry; cultivars; sterilization; multiplication; germplasm (en)
http://linked.open.../riv/klicoveSlovo	cultivars highbush blueberry sterilization germplasm multiplication lingonberry
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[A925D386D795]
http://linked.open...i/riv/nazevZdroje	Agriculture
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Research on less known fruit crops and their introduction to agrarian systems.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Paprštein, František Sedlák, Jiří
issn	0551-3677
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Maintenance of Vaccinium gene resources in actively growing in vitro cultures Maintenance of Vaccinium gene resources in actively growing in vitro cultures Maintenance of Vaccinium gene resources in actively growing in vitro cultures Maintenance of Vaccinium gene resources in actively growing in vitro cultures

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