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Description
  • A viroid disease syndrome that was recently described on Slovenian cultivar Celeia was investigated. The disease was transferred using biolistic approach to Czech hop under isolated experimental conditions. A complex of strong symptoms appeared on cultivar Osvald’s 72 that was quite comparable to cultivar Celeia; low plant fitness strong stunting, leaf malformation, small (tiny) leaves and frequently visible leaf mosaic, which was not associated with virus infection. Hop stunt viroid (HpSVd) was analyzed in detail in the inoculated Czech hop that survived initial infection. It was confirmed that a population of viroid was detectable in the leaves with a clearly dominant HpSVd-cucumber pale fruit viroid-like (HpSVd-cuc) sequence. Besides HpSVd, also traces of truncated Citrus viroid (CVd IV) were detected in surviving biolistically infected hop samples. In some samples, HpSVd was detectable by Northern blots, but not by RT PCR, suggesting some viroid sequence change(s) upon adaptation in hop. An expression of several pathogenesis-associated marker genes were investigated. It was found that experimentally induced viroid syndrome in Osvald’s 72 led to gene deregulation and especially to strong depression of HlbHLH2 transcription factor participating in metabolome regulation in hop.
  • A viroid disease syndrome that was recently described on Slovenian cultivar Celeia was investigated. The disease was transferred using biolistic approach to Czech hop under isolated experimental conditions. A complex of strong symptoms appeared on cultivar Osvald’s 72 that was quite comparable to cultivar Celeia; low plant fitness strong stunting, leaf malformation, small (tiny) leaves and frequently visible leaf mosaic, which was not associated with virus infection. Hop stunt viroid (HpSVd) was analyzed in detail in the inoculated Czech hop that survived initial infection. It was confirmed that a population of viroid was detectable in the leaves with a clearly dominant HpSVd-cucumber pale fruit viroid-like (HpSVd-cuc) sequence. Besides HpSVd, also traces of truncated Citrus viroid (CVd IV) were detected in surviving biolistically infected hop samples. In some samples, HpSVd was detectable by Northern blots, but not by RT PCR, suggesting some viroid sequence change(s) upon adaptation in hop. An expression of several pathogenesis-associated marker genes were investigated. It was found that experimentally induced viroid syndrome in Osvald’s 72 led to gene deregulation and especially to strong depression of HlbHLH2 transcription factor participating in metabolome regulation in hop. (en)
Title
  • Biolistic transfer of hop viroid disease syndrome from Slovenian cultivar Celeia to Czech hop Osvald's 72: pathogenesis symptoms and identification of dominant sequence upon transfer of HSVd component
  • Biolistic transfer of hop viroid disease syndrome from Slovenian cultivar Celeia to Czech hop Osvald's 72: pathogenesis symptoms and identification of dominant sequence upon transfer of HSVd component (en)
skos:prefLabel
  • Biolistic transfer of hop viroid disease syndrome from Slovenian cultivar Celeia to Czech hop Osvald's 72: pathogenesis symptoms and identification of dominant sequence upon transfer of HSVd component
  • Biolistic transfer of hop viroid disease syndrome from Slovenian cultivar Celeia to Czech hop Osvald's 72: pathogenesis symptoms and identification of dominant sequence upon transfer of HSVd component (en)
skos:notation
  • RIV/14864347:_____/13:#0000501!RIV14-MZE-14864347
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA13-03037S), P(QH81052)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 63460
http://linked.open...ai/riv/idVysledku
  • RIV/14864347:_____/13:#0000501
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • hop (Humulus lupulus L.); viroid symptoms (Pospiviroids); transcription factor and gene deregulation; Real Time quantitative RT-PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [995180FC5E28]
http://linked.open...v/mistoKonaniAkce
  • Žatec, ČR
http://linked.open...i/riv/mistoVydani
  • Leuven, Belgie
http://linked.open...i/riv/nazevZdroje
  • Acta Horticulturae 1010
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Matoušek, Jaroslav
  • Svoboda, Petr
  • Patzak, Josef
  • Duraisamy, G. S.
  • Orctová, Lidmila
  • Jakse, Jernej
  • Radisek, Sebastijan
  • Rausche, J.
  • Uhlířová, Karolína
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
issn
  • 0567-7572
number of pages
http://purl.org/ne...btex#hasPublisher
  • International Society for Horticultural Science (ISHS)
https://schema.org/isbn
  • 978-90-6605-696-1
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