About: Study of molecular markers for xanthohumol and DMX contents in hop (Humulus lupulus L.) by QTLs mapping analysis.     Goto   Sponge   Distinct   Permalink

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  • Recently, a medicinal usage of hop metabolic compounds is of increasing interest, mainly prenylflavonoids: xanthohumol (X) and desmethylxanthohumol (DMX). Quantitative trait loci (QTL) mapping is a good tool for finding reliable molecular markers for prospective breeding. We used STS, SSR, EST-SSR and AFLP molecular markers for analysing 116 individual female F1 hop genotypes. A total of 106 markers were used to construct the genetic linkage maps for female parent Taurus and male parent H06/14. In QTL analysis, 14 putative QTL molecular markers for xanthohumol content, 8 putative QTL molecular markers for DMX content and 8 putative QTL molecular for bitter acid content were detected. A QTL marker for an allele of the O-methyltransferase (omt1) gene confirmed the negative correlation between xanthohumol and DMX contents caused by final methylation. The other QTL molecular markers for alleles of chalcone synthase-like genes (vps, chs3, and chs4), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), geranylpyrophosphate synthase (gpps) and leucoanthocyanidin reductase 1 (lar1) genes showed the interconnection of bitter acids, terpenes and other polyphenols biosynthetic pathways. The reliability of QTL markers was proven by correlation analyses in 68 world hop cultivars.
  • Recently, a medicinal usage of hop metabolic compounds is of increasing interest, mainly prenylflavonoids: xanthohumol (X) and desmethylxanthohumol (DMX). Quantitative trait loci (QTL) mapping is a good tool for finding reliable molecular markers for prospective breeding. We used STS, SSR, EST-SSR and AFLP molecular markers for analysing 116 individual female F1 hop genotypes. A total of 106 markers were used to construct the genetic linkage maps for female parent Taurus and male parent H06/14. In QTL analysis, 14 putative QTL molecular markers for xanthohumol content, 8 putative QTL molecular markers for DMX content and 8 putative QTL molecular for bitter acid content were detected. A QTL marker for an allele of the O-methyltransferase (omt1) gene confirmed the negative correlation between xanthohumol and DMX contents caused by final methylation. The other QTL molecular markers for alleles of chalcone synthase-like genes (vps, chs3, and chs4), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF), geranylpyrophosphate synthase (gpps) and leucoanthocyanidin reductase 1 (lar1) genes showed the interconnection of bitter acids, terpenes and other polyphenols biosynthetic pathways. The reliability of QTL markers was proven by correlation analyses in 68 world hop cultivars. (en)
Title
  • Study of molecular markers for xanthohumol and DMX contents in hop (Humulus lupulus L.) by QTLs mapping analysis.
  • Study of molecular markers for xanthohumol and DMX contents in hop (Humulus lupulus L.) by QTLs mapping analysis. (en)
skos:prefLabel
  • Study of molecular markers for xanthohumol and DMX contents in hop (Humulus lupulus L.) by QTLs mapping analysis.
  • Study of molecular markers for xanthohumol and DMX contents in hop (Humulus lupulus L.) by QTLs mapping analysis. (en)
skos:notation
  • RIV/14864347:_____/12:#0000379!RIV13-MZE-14864347
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
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  • P(2B06011), P(QH81052)
http://linked.open...iv/cisloPeriodika
  • 7/8
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  • 172184
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  • RIV/14864347:_____/12:#0000379
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • hop (Humulus lupulus); QTL mapping; molecular markers (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [FC7FAB3FD344]
http://linked.open...i/riv/nazevZdroje
  • Brewing Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 65
http://linked.open...iv/tvurceVysledku
  • Krofta, Karel
  • Nesvadba, Vladimír
  • Patzak, Josef
  • Henychová, Alena
issn
  • 1613-2041
number of pages
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