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| - A new capillary gas chromatographic assay with nitrogen phosphorus detection for the determination of topiramate in the serum was developed and validated. The sample procedure included a liquid-liquid extraction of 0.1 mL of alkalized sample with ethyl acetate. The organic solvent was evaporated, and flash methylation of topiramate and internal standard 5-(p-methylphenyl)-5-phenylhydantoin with trimethylanilinium hydroxide (0.07 mol L-1 in methanol) was performed. The structure of derivatization product was confirmed using gas chromatography-mass spectrometry. Validation experiments certified suitability of bioanalytical method in the monitoring of serum concentrations in epileptic patients. The limit of detection was found to be 1.69 mu mol L-1. The range of applicability and linearity was established from 4.35 to 69.62 mu mol L-1. The accuracy and precision reached acceptable values from 86.15 to 109.5% (recovery) respectively, values were from 2.19 to 11.03% (RSD). No interference w
- A new capillary gas chromatographic assay with nitrogen phosphorus detection for the determination of topiramate in the serum was developed and validated. The sample procedure included a liquid-liquid extraction of 0.1 mL of alkalized sample with ethyl acetate. The organic solvent was evaporated, and flash methylation of topiramate and internal standard 5-(p-methylphenyl)-5-phenylhydantoin with trimethylanilinium hydroxide (0.07 mol L-1 in methanol) was performed. The structure of derivatization product was confirmed using gas chromatography-mass spectrometry. Validation experiments certified suitability of bioanalytical method in the monitoring of serum concentrations in epileptic patients. The limit of detection was found to be 1.69 mu mol L-1. The range of applicability and linearity was established from 4.35 to 69.62 mu mol L-1. The accuracy and precision reached acceptable values from 86.15 to 109.5% (recovery) respectively, values were from 2.19 to 11.03% (RSD). No interference w (en)
- Vyvinuli jsme novou chromatografickou metodu s termionickým detektorem pro stanovení topiramátu v séru. Topiramát byl extrahován z 0,1 ml alkalizovaného séra etylacetátem. Organická vrstva se odpařila a provedla se metylace topiramátu a interního standardu 5-(p-methylphenyl)-5-phenylhydantoinu trimethylanilinium hydroxidem (0.07 mol L-1 v methanolu). Na potvrzení struktury derivatizovaných produktů jsme použili plynovou chromatografii s hmotnostní detekcí. Validace metody potvrdila její vhodnost pro monitorování sérových koncentrací topiramátu u epileptických pacientů. Limit detekce byl 1.69 mu mol L-1. Linearita byla stanovena od 4.35 do 69.62. Přesnost dosáhla akceptovatelných hodnot od 86.15 do 109.5% a variační koeficient byl od 2.19 do 11.03%. (cs)
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skos:notation
| - RIV/00843989:_____/07:00014895!RIV09-MZ0-00843989
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00843989:_____/07:00014895
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - gas chromatography; derivatization; topiramate (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Brozmanová, Hana
- Maláková, J.
- Palicka, V.
- Prochazkova, V.
- Vorisek, V.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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