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  • Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance liquid chromatography method for the determination of retinol and alpha-tocopherol in human seminal plasma was developed.
  • Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance liquid chromatography method for the determination of retinol and alpha-tocopherol in human seminal plasma was developed. (en)
Title
  • Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection
  • Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection (en)
skos:prefLabel
  • Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection
  • Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection (en)
skos:notation
  • RIV/00216275:25310/14:39898252!RIV15-MSM-25310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
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  • 10812
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  • RIV/00216275:25310/14:39898252
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  • tocopherol; seminal plasma; retinol; high-performance liquid chromatography; Butylated hydroxytoluene (en)
http://linked.open.../riv/klicoveSlovo
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  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [C7AA106F1BFA]
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  • Andrologia
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  • 46
http://linked.open...iv/tvurceVysledku
  • Kanďár, Roman
  • Drábková, Petra
  • Hampl, Radek
  • Myslíková, Kateřina
http://linked.open...ain/vavai/riv/wos
  • 000335009600003
issn
  • 0303-4569
number of pages
http://bibframe.org/vocab/doi
  • 10.1111/and.12103
http://localhost/t...ganizacniJednotka
  • 25310
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