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  • Normal-phase liquid chromatography is important complement to more frequently used reversed-phase liquid chromatography. It can be performed in fully organic mobile phases (adsorption liquid chromatography), where it offers advantages for separation of low-molecular moderately polar samples based on the differences in the number and position of functional groups. It allows large variability in selectivity by selection of appropriate mobile phase. Aqueous normal-phase chromatography is rapidly developing separation technique suitable for separation of both moderately and strongly polar (even ionic) compounds, which are difficult to be accomplished in other separation systems. A plethora of polar stationary phases are available for separations in aqueous-organic mobile phases, some of which provide dual retention mechanism and can be used either in the normal-phase mode in organic rich mobile phases, or in the reversed-phase mode in highly aqueous mobile phases.
  • Normal-phase liquid chromatography is important complement to more frequently used reversed-phase liquid chromatography. It can be performed in fully organic mobile phases (adsorption liquid chromatography), where it offers advantages for separation of low-molecular moderately polar samples based on the differences in the number and position of functional groups. It allows large variability in selectivity by selection of appropriate mobile phase. Aqueous normal-phase chromatography is rapidly developing separation technique suitable for separation of both moderately and strongly polar (even ionic) compounds, which are difficult to be accomplished in other separation systems. A plethora of polar stationary phases are available for separations in aqueous-organic mobile phases, some of which provide dual retention mechanism and can be used either in the normal-phase mode in organic rich mobile phases, or in the reversed-phase mode in highly aqueous mobile phases. (en)
Title
  • Liquid chromatography - Normal phase
  • Liquid chromatography - Normal phase (en)
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  • Liquid chromatography - Normal phase
  • Liquid chromatography - Normal phase (en)
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  • RIV/00216275:25310/13:39897450!RIV14-MSM-25310___
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  • RIV/00216275:25310/13:39897450
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  • Stationary phase; Normal phase; Mobile phase; Liquid chromatography; HPLC; HILIC; Aqueous normal phase; Adsorption chromatography (en)
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  • [07CAED84301A]
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  • Reference Module in Chemistry, Molecular Sciences and Chemical Engineering
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  • Jandera, Pavel
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  • Elsevier Science
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  • 978-0-12-409547-2
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  • 25310
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