About: Is it correct to leave out the fluorimetric methods for glutathione assay?

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Glutathione is the main intracellular antioxidant, which exists in two forms, the reduced and the oxidized. Changes in glutathione concentration serve as an important marker of the redox state inside the cells. Various methods can be used to measure glutathione concentration in biological material, namely enzymatic, fluorimetric or HPLC. Nevertheless, due to the high sensitivity and specificity HPLC methods are preferred to analyze glutathione concentration, in spite of high price and time demand. The aim of our study was to establish analytical parameters of the spectrofluorimetric method, which should be suitable for the analysis of huge amount of samples. This method is based on the well-known principle - reaction between glutathione and o phthaldialdehyde with subsequent production of strongly fluorescent isoindole-like product. To confirm selectivity of this method, we have analysed glutathione concentration in hepatocyte lysates using HPLC method with spectrofluorimetric detection. Hepatocytes a Glutathione is the main intracellular antioxidant, which exists in two forms, the reduced and the oxidized. Changes in glutathione concentration serve as an important marker of the redox state inside the cells. Various methods can be used to measure glutathione concentration in biological material, namely enzymatic, fluorimetric or HPLC. Nevertheless, due to the high sensitivity and specificity HPLC methods are preferred to analyze glutathione concentration, in spite of high price and time demand. The aim of our study was to establish analytical parameters of the spectrofluorimetric method, which should be suitable for the analysis of huge amount of samples. This method is based on the well-known principle - reaction between glutathione and o phthaldialdehyde with subsequent production of strongly fluorescent isoindole-like product. To confirm selectivity of this method, we have analysed glutathione concentration in hepatocyte lysates using HPLC method with spectrofluorimetric detection. Hepatocytes a (en) V práci byly stanoveny analytické parametry spektrofluorimetrického stanovení glutathionu, které prokázaly vhodnost metody pro stanovení glutathionu v biologickém materiálu. (cs)
Title	Is it correct to leave out the fluorimetric methods for glutathione assay? Je správné opomíjet fluorimetrické metody při stanovení glutathionu? (cs) Is it correct to leave out the fluorimetric methods for glutathione assay? (en)
skos:prefLabel	Is it correct to leave out the fluorimetric methods for glutathione assay? Je správné opomíjet fluorimetrické metody při stanovení glutathionu? (cs) Is it correct to leave out the fluorimetric methods for glutathione assay? (en)
skos:notation	RIV/00216275:25310/05:00003016!RIV08-MSM-25310___
http://linked.open.../vavai/riv/strany	207-208
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM0021627502)
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Roušar, Tomáš Kanďár, Roman Červinková, Zuzana Mužáková, Vladimíra
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Pardubice / Fakulta chemicko-technologická
http://linked.open...dnocenehoVysledku	525699
http://linked.open...ai/riv/idVysledku	RIV/00216275:25310/05:00003016
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	glutathione; fluorimetric assay; antioxidants (en)
http://linked.open.../riv/klicoveSlovo	antioxidants glutathione fluorimetric assay
http://linked.open...ontrolniKodProRIV	[ABB4EEB8EA06]
http://linked.open...v/mistoKonaniAkce	Glasgow, Skotsko
http://linked.open...i/riv/mistoVydani	UK
http://linked.open...i/riv/nazevZdroje	Clin. Chim. Acta suppl.
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Kanďár, Roman Roušar, Tomáš Kučera, Otto Lotková, Halka Červinková, Zuzana Mužáková, Vladimíra
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2005-05-08 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Pokročilé analytické a separační metody a jejich aplikace v diagnostice a technologii živých a neživých materiálů
issn	0009-8981
number of pages	2 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Elsevier
http://localhost/t...ganizacniJednotka	25310
is http://linked.open...avai/riv/vysledek of	Is it correct to leave out the fluorimetric methods for glutathione assay?

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