About: Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis     Goto   Sponge   Distinct   Permalink

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Description
  • A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6.
  • A novel approach for diagnosis of cystic fibrosis is presented. A simple and fast procedure to obtain sweat sample was developed. It consists of repeatedly wiping the skin of the forearm with deionized water moisturized cotton swab and extraction in 1 mL of deionized water. Double opposite end injection capillary electrophoresis with contactless conductometric detection is used for the analysis of the extract. Chloride, sodium and potassium as the three target ions that participate in the ion transfer across the cellular membranes, and are affected by CF, are simultaneously determined in approximately 3 min in a background electrolyte containing 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine and 2 mM 18-crown-6. (en)
Title
  • Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
  • Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis (en)
skos:prefLabel
  • Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis
  • Double opposite end injection capillary electrophoresis with contactless conductometric detection for simultaneous determination of chloride, sodium and potassium in cystic fibrosis diagnosis (en)
skos:notation
  • RIV/00216224:14740/14:00074081!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED1.1.00/02.0068), P(GA13-21919S)
http://linked.open...iv/cisloPeriodika
  • September
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 12300
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14740/14:00074081
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Capillary electrophoresis; Contactless conductivity detection; Ions; Cystic fibrosis; Principal component analysis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [13DE9C213F39]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography A
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1358
http://linked.open...iv/tvurceVysledku
  • Foret, František
  • Skřičková, Jana
  • Pokojová, Eva
  • Kubáň, Petr
  • Greguš, Michal
http://linked.open...ain/vavai/riv/wos
  • 000340979200035
issn
  • 0021-9673
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.chroma.2014.06.091
http://localhost/t...ganizacniJednotka
  • 14740
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