About: New sensitive detection method for lectin hemagglutination using microscopy.     Goto   Sponge   Distinct   Permalink

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Description
  • The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope.
  • The blood group system AB0 is determined by the composition of terminal oligosaccharides on red blood cells. Thanks to this structural feature, these groups can be recognized by saccharide-recognizing compounds. Hemagglutination is a process in which blood cells are cross-linked via multivalent molecules, lectins e.g. A hemagglutination assay is commonly used for the detection of multivalent molecules that recognize blood cells, in order to search for their sugar specificity. It is traditionally performed on a microtiter plate, where the lectin solution is serially diluted and the lowest concentration of lectin causing agglutination is detected. We have developed a new way of detecting hemagglutination using microscopy, which was tested on purified lectins as well as cell lysates. Hemagglutination was performed on a microscope slide directly and detected using a microscope. (en)
Title
  • New sensitive detection method for lectin hemagglutination using microscopy.
  • New sensitive detection method for lectin hemagglutination using microscopy. (en)
skos:prefLabel
  • New sensitive detection method for lectin hemagglutination using microscopy.
  • New sensitive detection method for lectin hemagglutination using microscopy. (en)
skos:notation
  • RIV/00216224:14740/14:00073809!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED1.1.00/02.0068), P(EE2.3.30.0009), P(GA13-25401S)
http://linked.open...iv/cisloPeriodika
  • 10
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 32491
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14740/14:00073809
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • hemagglutination; protein-carbohydrate interactions; blood group antigens; lectin (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [384FBAADA719]
http://linked.open...i/riv/nazevZdroje
  • Microscopy research and technique
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 77
http://linked.open...iv/tvurceVysledku
  • Adamová, Lenka
  • Malinovská, Lenka
  • Wimmerová, Michaela
http://linked.open...ain/vavai/riv/wos
  • 000342207900012
issn
  • 1059-910X
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/jemt.22407
http://localhost/t...ganizacniJednotka
  • 14740
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