About: Alternative Reliable Method for Cytochrome P450 2D6 Poor Metabolizers Genotyping

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/article/10.1007%2Fs12033-012-9510-2
Description	High-resolution melting curve analysis (HRM) of polymerase chain reaction (PCR) amplicons has been described as a fast, cheap and reliable closed-tube method of genotyping with no need for labeled primers or labeled probes. We adapted this melting analysis assay for the detection of the most common nonfunctional alleles of cytochrome P-450 (CYP) 2D6 in the Caucasian population that affect the metabolism of many commonly used drugs. We used this method to genotype 91 patients under paroxetine therapy. The presence and constitution of the most common single-nucleotide polymorphisms (1846G>A, 2988G>A, 100C>T, 2549delA, 2615_2617delAAG, 1707delT) in poor and intermediate metabolizers from the Caucasian population were detected in short amplicons 148 bp). After fluorescence normalization, the wild-type, homozygous and heterozygous samples were easily distinguishable from each other by their specific melting curve shape. A total of 92. High-resolution melting curve analysis (HRM) of polymerase chain reaction (PCR) amplicons has been described as a fast, cheap and reliable closed-tube method of genotyping with no need for labeled primers or labeled probes. We adapted this melting analysis assay for the detection of the most common nonfunctional alleles of cytochrome P-450 (CYP) 2D6 in the Caucasian population that affect the metabolism of many commonly used drugs. We used this method to genotype 91 patients under paroxetine therapy. The presence and constitution of the most common single-nucleotide polymorphisms (1846G>A, 2988G>A, 100C>T, 2549delA, 2615_2617delAAG, 1707delT) in poor and intermediate metabolizers from the Caucasian population were detected in short amplicons 148 bp). After fluorescence normalization, the wild-type, homozygous and heterozygous samples were easily distinguishable from each other by their specific melting curve shape. A total of 92. (en)
Title	Alternative Reliable Method for Cytochrome P450 2D6 Poor Metabolizers Genotyping Alternative Reliable Method for Cytochrome P450 2D6 Poor Metabolizers Genotyping (en)
skos:prefLabel	Alternative Reliable Method for Cytochrome P450 2D6 Poor Metabolizers Genotyping Alternative Reliable Method for Cytochrome P450 2D6 Poor Metabolizers Genotyping (en)
skos:notation	RIV/00216224:14740/13:00065563!RIV14-MZ0-14740___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(NS9676)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Pavelka, Antonín Juřica, Jan Žourková, Alexandra Pindurová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	60171
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/13:00065563
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	High resolution melting analysis; CYP2D6; poor metabolizers; SNP; short amplicon; melting curve (en)
http://linked.open.../riv/klicoveSlovo	SNP High resolution melting analysis poor metabolizers short amplicon CYP2D6 melting curve
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[BDA57A1C9F4A]
http://linked.open...i/riv/nazevZdroje	Molecular Biotechnology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology The influence of pharmacogenetic and pharmacokinetic factors on the efficacy and safety of the first schizophrenia episodes therapy
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Juřica, Jan Pavelka, Antonín Pindurová, Eva Zrůstová, Jana Žourková, Alexandra
http://linked.open...ain/vavai/riv/wos	000312640400004
issn	1073-6085
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12033-012-9510-2
http://localhost/t...ganizacniJednotka	14740

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