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Description
| - Crystal structure of novel haloalkane dehalogenase DbeA revealed the presence of two chloride ions buried in the protein interior. The first halide-binding site is involved in substrate binding and is present in all structurally characterized haloalkane dehalogenases. The second halide-binding site is unique to DbeA. To elucidate the role of the second halide-binding site in enzyme functionality, a two-point mutant lacking this site was constructed and characterized. These substitutions resulted in a shift of substrate-specificity class and were accompanied by decrease of enzyme activity, stability and elimination of the substrate inhibition. The changes in enzyme catalytic activity were attributed to deceleration of the rate-limiting hydrolytic step, mediated by lower basicity of the catalytic histidine.
- Crystal structure of novel haloalkane dehalogenase DbeA revealed the presence of two chloride ions buried in the protein interior. The first halide-binding site is involved in substrate binding and is present in all structurally characterized haloalkane dehalogenases. The second halide-binding site is unique to DbeA. To elucidate the role of the second halide-binding site in enzyme functionality, a two-point mutant lacking this site was constructed and characterized. These substitutions resulted in a shift of substrate-specificity class and were accompanied by decrease of enzyme activity, stability and elimination of the substrate inhibition. The changes in enzyme catalytic activity were attributed to deceleration of the rate-limiting hydrolytic step, mediated by lower basicity of the catalytic histidine. (en)
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Title
| - Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites.
- Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites. (en)
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skos:prefLabel
| - Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites.
- Structural and Functional Analysis of a Novel Haloalkane Dehalogenase with Two Halide-Binding Sites. (en)
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skos:notation
| - RIV/00216224:14310/14:00074206!RIV15-MSM-14310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - I, P(GAP207/12/0775), P(LO1214), S
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/14:00074206
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - haloalkane dehalogenase DbeA from Bradyrhizobium elkanii USDA94 (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Brezovský, Jan
- Chaloupková, Radka
- Damborský, Jiří
- Koudeláková, Táňa
- Prokop, Zbyněk
- Nagata, Y.
- Prudnikova, T.
- Sato, Y.
- Kuta Smatanova, I.
- Kuty, M.
- Rezacova, P.
- Daniel, Lukáš
- Ikeda-Ohtsubo, W.
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
| - 10.1107/S1399004714009018
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http://localhost/t...ganizacniJednotka
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