About: Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.

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About: Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. Goto Sponge Distinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid (aa) residues, exhibit only seven amino acid residue differences between them, but LinBMI´s catalytic performance towards beta-hexachlorocyclohexane (beta-HCH) is considerably higher than LinBUT´s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT´s b-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations. Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid (aa) residues, exhibit only seven amino acid residue differences between them, but LinBMI´s catalytic performance towards beta-hexachlorocyclohexane (beta-HCH) is considerably higher than LinBUT´s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT´s b-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations. (en)
Title	Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. (en)
skos:prefLabel	Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. (en)
skos:notation	RIV/00216224:14310/14:00074201!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP503/12/0572), P(LO1214)
http://linked.open...iv/cisloPeriodika	72
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Prokop, Zbyněk Damborský, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	47494
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/14:00074201
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	beta -Hexachlorocyclohexane; Xenobiotics; Biodegradation; Haloalkane dehalogenase; Protein evolution (en)
http://linked.open.../riv/klicoveSlovo	Protein evolution beta -Hexachlorocyclohexane Xenobiotics Biodegradation Haloalkane dehalogenase
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[BCFAF4555C5D]
http://linked.open...i/riv/nazevZdroje	Applied Microbiology and Biotechnology Express
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Construction of a synthetic metabolic pathway for degradation of important environmental pollutant using protein and metabolic engineering Centrum pro výzkum toxických látek v prostředí
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	4
http://linked.open...iv/tvurceVysledku	Damborský, Jiří Ishitsuka, M. Prokop, Zbyněk Tanaka, H. Nagata, Y. Ito, M. Moriuchi, R. Nikawadori, Y. Ohtsubo, Y. Tsuda, M.
issn	2191-0855
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1186/s13568-014-0072-5
http://localhost/t...ganizacniJednotka	14310

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