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  • Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system.
  • Four types of coating procedures suitable for protein and peptides separation: covalent coating using polyethyleneimine (PEI), 3-glycidoxy-propyltrimethoxysilane (GPTMS), 3-aminopropyltriethoxysilane (APS) and a new approach of dynamic coating with linear polyacrylamide (LPA) have been studied. The capillary surface was characterized by determination of the electroosmotic flow (EOF); for optimization a model mixture of proteins (cytochrome C, lysozyme and ribonuclease A) was used. Separation of a neuroprotective peptide [Gly-14]humanin was demonstrated in the optimized system. (en)
Title
  • Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
  • Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides (en)
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  • Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides
  • Minimization of adsorption in capillary zone electrophoresis of proteins and neuroprotective peptides (en)
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  • RIV/00216224:14310/05:00020042!RIV10-GA0-14310___
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  • P(GA305/03/1100)
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  • 530354
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  • RIV/00216224:14310/05:00020042
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  • Capillary electrophoresis; Coating; Electroosmotic flow; Polyethyleneimine; 3glycidoxypropyltrimethoxysilane; 3aminopropyltriethoxysilane; Linear polyacrylamide (en)
http://linked.open.../riv/klicoveSlovo
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  • [235ECF6F3667]
http://linked.open...v/mistoKonaniAkce
  • Prague, Czech republic
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  • Prague, Czech republic
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  • Collection Symposium Series, Biologically Active Peptides
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  • Bittová, Miroslava
  • Havel, Josef
  • Pazourek, Jiří
  • Šulák, Ondřej
  • Gajdošová, Dagmar
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number of pages
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  • Ústav organické chemie a biochemie AV ČR
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  • 80-86241-26-2
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  • 14310
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