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  • Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and PCR methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells.
  • Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and PCR methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells. (en)
  • Magnetic nonporous poly(HEMA-co-EDMA) and poly(HEMA-co-GMA) microspheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of magnetite. They were functionalized by polyclonal Salmonella antibodies via the trichlorotriazine method. Salmonella cells were then successfully identified using cultural and PCR methods after their immunomagnetic separation. The PCR sensitivity of target cell detection was negatively influenced by the presence of some compounds used in the process of particle preparation. In some cases, magnetic poly(HEMA-co-EDMA) microspheres with immobilized proteinase K were used for degradation of intracellular inhibitors present in Salmonella cells. (cs)
Title
  • Immunomagnetic separation of Salmonella cells using newly designed carriers
  • Immunomagnetic separation of Salmonella cells using newly designed carriers (en)
  • Immunomagnetic separation of Salmonella cells using newly designed carriers (cs)
skos:prefLabel
  • Immunomagnetic separation of Salmonella cells using newly designed carriers
  • Immunomagnetic separation of Salmonella cells using newly designed carriers (en)
  • Immunomagnetic separation of Salmonella cells using newly designed carriers (cs)
skos:notation
  • RIV/00216224:14310/03:00008145!RIV08-GA0-14310___
http://linked.open.../vavai/riv/strany
  • 215-221
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/00/1339)
http://linked.open...iv/cisloPeriodika
  • 1-2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 609838
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/03:00008145
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Salmonella spp. magnetic sorbents; immobilization; affinity sorbents; hydroxyethyl methacrylate; glycidyl methacrylate; ethylene methacrylate; proteinase K; enzymes; antibodies (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [70C9CF640895]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography A
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1009
http://linked.open...iv/tvurceVysledku
  • Horák, Daniel
  • Rittich, Bohuslav
  • Španová, Alena
  • Lenfeld, Jiří
  • Prodělalová, Jana
  • Sučiková, Jana
  • Štrumcová, Soňa
issn
  • 0021-9673
number of pages
http://localhost/t...ganizacniJednotka
  • 14310
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