About: A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is sug The cells of Saccharomyces cerevisiae were exposed to the effect of mild and lethal heat stress and their viability and changes in their microtubular and actin structures were studied. An increase in cultivation temperature from 25 C to 46 C (lethal heat shock, LHS) resulted, during 10 min, in a rapid decrease in cell viability. However, a mild increase in incubation temperature from 25 C to 37 C or 41 C (mild heat shock, MHS) induced cell tolerance to LHS and the cells were able to survive at 46 C for up to 30 min. A mild heat shock increased the level of Hsp104 in cells, as demonstrated by western blot analysis and indirect immunofluorescence microscopy. In cell exposed to LHS, immunofluorescence microscopy showed rapid degradation of both cytoplasmic and nuclear microtubules, but the spindle pole bodies (SPB) remained preserved. On the other hand, MHS did not produce microtubule degradation. Microtubules remained stable after the LHS that had been preceded by cell cultivation at 37 C. It is sug (en)
Title	A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures (en)
skos:prefLabel	A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures A response of yeast cells to heat stress: cell viability and the stabilitz of cytoskeletal structures (en)
skos:notation	RIV/00216224:14110/00:00005108!RIV/2002/GA0/141102/N
http://linked.open.../vavai/riv/strany	381
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA204/00/0394)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Müller, Petr Holubářová, Alena Svoboda, Augustin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	703691
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/00:00005108
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Saccharomyces cerevisiae, heat shock, Hsp104, actin, microtubules (en)
http://linked.open.../riv/klicoveSlovo	actin heat shock Saccharomyces cerevisiae microtubules Hsp104
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[0321594EAD07]
http://linked.open...i/riv/nazevZdroje	Scripta medica
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	73
http://linked.open...iv/tvurceVysledku	Müller, Petr Svoboda, Augustin Holubářová, Alena
issn	1211-3395
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

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