About: Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1039/c4pp00121d
Description	A new setup for direct microspectroscopic monitoring of singlet oxygen (O-1(2)) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect O-1(2). The O-1(2') phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of O-1(2) phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence. A new setup for direct microspectroscopic monitoring of singlet oxygen (O-1(2)) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect O-1(2). The O-1(2') phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of O-1(2) phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence. (en)
Title	Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells (en)
skos:prefLabel	Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells (en)
skos:notation	RIV/00216208:11320/14:10286185!RIV15-MSM-11320___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(GBP501/12/G055), S
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Valenta, Jan Dědic, Roman Hála, Jan Scholz, Marek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	41497
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/14:10286185
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ccd; lifetime; diffusion; sensitizer; in-vivo; optical-detection; delayed fluorescence; water-soluble photosensitizers (en)
http://linked.open.../riv/klicoveSlovo	delayed fluorescence lifetime sensitizer diffusion in-vivo ccd optical-detection water-soluble photosensitizers
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[34FCCD5D9868]
http://linked.open...i/riv/nazevZdroje	Photochemical and Photobiological Sciences
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Photosynthesis Research Center
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	13
http://linked.open...iv/tvurceVysledku	Valenta, Jan Dědic, Roman Hála, Jan Scholz, Marek Breitenbach, Thomas
http://linked.open...ain/vavai/riv/wos	000341296500012
issn	1474-905X
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1039/c4pp00121d
http://localhost/t...ganizacniJednotka	11320

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