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  • The aim of this study was to modify the isolation procedure by excluding Ficoll gradient centrifugation to obtain a similar or better yield of viable, functional islets. In our modification of the isolation procedure, the separation of islets from exocrine tissue was based on their sedimentation rate combined with their differential ability to attach to the surface of culture dishes for suspension cells. The mean yield was 900 viable, insulin-producing islets per mouse, which was comparable to or even higher than the yield in commonly used protocols
  • The aim of this study was to modify the isolation procedure by excluding Ficoll gradient centrifugation to obtain a similar or better yield of viable, functional islets. In our modification of the isolation procedure, the separation of islets from exocrine tissue was based on their sedimentation rate combined with their differential ability to attach to the surface of culture dishes for suspension cells. The mean yield was 900 viable, insulin-producing islets per mouse, which was comparable to or even higher than the yield in commonly used protocols (en)
  • Cílem práce bylo modifikovat standardní metodu izolace Langerhansových ostrůvků vynecháním centrifugace ve Ficollovém gradientu, avšak s dosažením podobného nebo lepšího výtěžku živých, funkčních Langerhansových ostrůvků. V naší modifikaci izolační procedury dochází k separaci ostrůvků od exokrinní tkáně na základě jejich odlišné sedimentační rychlosti a jejich rozdílné schopnosti přisedat na kultivační plastik pro suspenzní buňky. Průměrný výtěžek naší metody byl 900 živých, insulin-produkujících ostrůvků/myš, což je výtěžek srovnatelný nebo dokonce vyšší než udávaný výtěžek běžně používaných izolačních protokolů (cs)
Title
  • Modified method for isolation of Langerhans islets from mice
  • Modifikovaná metoda izolace Langerhansových ostrůvků z myši (cs)
  • Modified method for isolation of Langerhans islets from mice (en)
skos:prefLabel
  • Modified method for isolation of Langerhans islets from mice
  • Modifikovaná metoda izolace Langerhansových ostrůvků z myši (cs)
  • Modified method for isolation of Langerhans islets from mice (en)
skos:notation
  • RIV/00216208:11120/08:00000909!RIV09-MSM-11120___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM0021620814)
http://linked.open...iv/cisloPeriodika
  • 10
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 380225
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11120/08:00000909
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • isolation of Langerhans islets; mice (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [14BABB07B5B1]
http://linked.open...i/riv/nazevZdroje
  • Transplantation Proceedings
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 40
http://linked.open...iv/tvurceVysledku
  • Kovář, Jan
  • Fürstová, Vlasta
  • Kopská, Tereza
http://linked.open...ain/vavai/riv/wos
  • 000262133300079
http://linked.open...n/vavai/riv/zamer
issn
  • 0041-1345
number of pages
http://localhost/t...ganizacniJednotka
  • 11120
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