Attributes | Values |
---|
rdf:type
| |
Description
| - In this study, we develop the HPLC assay for the direct determination of alpha-tocopheryl succinate (TOS) entrapped in the liposomes as well as monitoring of TOS chemical stability in the liposomal lyophilisates during 6-month storage period. The composition of mobile phase was optimised for separation of TOS, alpha-tocopherol (TOH) as TOS degradation product and alpha-tocopheryl acetate as internal standard. The quantification of TOS in liposomal formulation and liposomal sediment enables to calculate entrappment efficiency of TOS (EETOS). For the liposomal lyophilisates stored during 6 months, the obtained values of EETOS were higher than 90%. The chemical stability of liposomal entrapped TOS was investigated by monitoring the level of TOH. Generally, the content of liposomal TOH did not exceed 5% of the liposomal TOS content.
- In this study, we develop the HPLC assay for the direct determination of alpha-tocopheryl succinate (TOS) entrapped in the liposomes as well as monitoring of TOS chemical stability in the liposomal lyophilisates during 6-month storage period. The composition of mobile phase was optimised for separation of TOS, alpha-tocopherol (TOH) as TOS degradation product and alpha-tocopheryl acetate as internal standard. The quantification of TOS in liposomal formulation and liposomal sediment enables to calculate entrappment efficiency of TOS (EETOS). For the liposomal lyophilisates stored during 6 months, the obtained values of EETOS were higher than 90%. The chemical stability of liposomal entrapped TOS was investigated by monitoring the level of TOH. Generally, the content of liposomal TOH did not exceed 5% of the liposomal TOS content. (en)
|
Title
| - Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate
- Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate (en)
|
skos:prefLabel
| - Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate
- Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate (en)
|
skos:notation
| - RIV/00027162:_____/10:#0000657!RIV11-AV0-00027162
|
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(KAN200520703), Z(MZE0002716202)
|
http://linked.open...iv/cisloPeriodika
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/10:#0000657
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - alpha-tocopheryl succinate; liposomes; stability; lyophilisation; high-performance liquid chromatography (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...odStatuVydavatele
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/nazevZdroje
| |
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...v/svazekPeriodika
| |
http://linked.open...iv/tvurceVysledku
| - Koudelka, Štěpán
- Mašek, Josef
- Turánek, Jaroslav
- Turánek-Knötigová, Pavlína
- Neužil, J.
|
http://linked.open...n/vavai/riv/zamer
| |
issn
| |
number of pages
| |
is http://linked.open...avai/riv/vysledek
of | |