About: Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this study was to determine whether apoptosis of lymphocytes is modulated by muramyl dipeptide (MDP) that is a minimal structural unit of peptidoglycan in Gram-positive bacteria. To this aim, apoptosis of lymphocytes was studied in in vitro environment (RPMI medium, 37 °C, 5% CO2) with 10 ug.ml-1 MDP. Heifers were used as mammary gland cell donors for in vitro studies. Lymphocytes from the intact mammary glands were harvested following the phosphate buffered saline intramammary injection. Apoptosis and necrosis of lymphocytes were analysed by flow cytometry following simultaneous staining with Annexin-V and propidium iodide. The results of this study demonstrate that apoptosis of lymphocytes was delayed during in vitro incubation with MDP. The aim of this study was to determine whether apoptosis of lymphocytes is modulated by muramyl dipeptide (MDP) that is a minimal structural unit of peptidoglycan in Gram-positive bacteria. To this aim, apoptosis of lymphocytes was studied in in vitro environment (RPMI medium, 37 °C, 5% CO2) with 10 ug.ml-1 MDP. Heifers were used as mammary gland cell donors for in vitro studies. Lymphocytes from the intact mammary glands were harvested following the phosphate buffered saline intramammary injection. Apoptosis and necrosis of lymphocytes were analysed by flow cytometry following simultaneous staining with Annexin-V and propidium iodide. The results of this study demonstrate that apoptosis of lymphocytes was delayed during in vitro incubation with MDP. (en)
Title	Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro (en)
skos:prefLabel	Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro (en)
skos:notation	RIV/00027162:_____/09:#0000558!RIV10-MZE-00027162
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	Suppl.1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Ryšánek, Dušan Sláma, Petr Sládek, Zbyšek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	312185
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/09:#0000558
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Apoptosis; lymphocyte; muramyl dipeptide; mammary gland (en)
http://linked.open.../riv/klicoveSlovo	lymphocyte Apoptosis mammary gland muramyl dipeptide
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[3F2D75B65213]
http://linked.open...i/riv/nazevZdroje	Slovak Journal of Animal Science
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	42
http://linked.open...iv/tvurceVysledku	Ryšánek, Dušan Sládek, Zbyšek Sláma, Petr
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	1337-9984
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro Effect of muramyl dipeptide on apoptosis of bovine mammary gland lymphocytes in vitro

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