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  • The development of a direct competitive enzyme-linked immunosorbent assay (ELISA) method based on the derivatization of semicarbazide (SEM) with o-nitrobenzaldehyde and on the immunochemical detection of the derivatited analyte is described. The small aliphatic molecule SEM was coupled to 3-carboxybenzaldehyde to produce carboxyphenyl-SEM (CPSEM), a larger hapten derivative for the generation of specific antibodies. Five rabbits produced antibodies against SEM exhibiting a 50% binding inhibition level (IC 50 value) of 0.06 to 2.28 ug /L in assay buffer. The superior indirect assay based on the antibody MVK39 showed a high dynamic range providing a linear readout in the range of 0.01 to 0.2 ug /L. The optimised ELISA based on MVK31 (IgG) was evaluated using solvent extracted SEM-spiked porcine and baby food puree samples.
  • The development of a direct competitive enzyme-linked immunosorbent assay (ELISA) method based on the derivatization of semicarbazide (SEM) with o-nitrobenzaldehyde and on the immunochemical detection of the derivatited analyte is described. The small aliphatic molecule SEM was coupled to 3-carboxybenzaldehyde to produce carboxyphenyl-SEM (CPSEM), a larger hapten derivative for the generation of specific antibodies. Five rabbits produced antibodies against SEM exhibiting a 50% binding inhibition level (IC 50 value) of 0.06 to 2.28 ug /L in assay buffer. The superior indirect assay based on the antibody MVK39 showed a high dynamic range providing a linear readout in the range of 0.01 to 0.2 ug /L. The optimised ELISA based on MVK31 (IgG) was evaluated using solvent extracted SEM-spiked porcine and baby food puree samples. (en)
  • Práce popisuje vývoj přímé kompetititvní ELISA (enzyme-linked immunosorbent assay) metody založené na derivatizaci semicarbazidu (SEM) with o-nitrobenzaldehyde a imunonochemické detekci tohoto derivatizovaného analytu. Malá alifatická molekula SEM byla kopulována s 3-karboxybenzaldehydem za tvorby karboxyphenyl-SEM (CPSEM), který je větším haptenovým derivatem pro produkci protilátek. Pět králíků produkovalo protilátky proti SEM vykázujíce 50% vazebnou inhibici v pufru na úrovni hodnot IC 50 od 0.06 do 2.28 ug / L. Nejlepší ELISA výsledky (v nepřímém formátu) byla dosažena s protilátkou MVK39, která ukázala vysoký lineární character kalibrace v rozsahu koncentrace 0.01 to 0.2 ug / L. Optimalizovaná ELISA pracující s MVK31 (IgG) protilátkou byla evaluována s použitím fortikovaných extraktů vzorků z hovězího masa a dětské výživy. (cs)
Title
  • ELISA for semicarbazide and its application for screening in food contamination
  • ELISA pro semicarbazid a její aplikace na screening kontaminace potravin (cs)
  • ELISA for semicarbazide and its application for screening in food contamination (en)
skos:prefLabel
  • ELISA for semicarbazide and its application for screening in food contamination
  • ELISA pro semicarbazid a její aplikace na screening kontaminace potravin (cs)
  • ELISA for semicarbazide and its application for screening in food contamination (en)
skos:notation
  • RIV/00027162:_____/08:#0000442!RIV09-MZE-00027162
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1B53020), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 365998
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/08:#0000442
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Nitrofurans; Hapten design; Semicarbazide detection; ELISA; Antibody specificity; Assay evaluation; porcine samples (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [11943CE22F40]
http://linked.open...i/riv/nazevZdroje
  • Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 608
http://linked.open...iv/tvurceVysledku
  • Diblíková, Iva
  • Fránek, Milan
  • Černoch, Ivo
  • Vass, Maria
http://linked.open...ain/vavai/riv/wos
  • 000253088800009
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-2670
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