About: In vitro induction of polyploidy in yacon (Smallanthus sonchifolius)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In vitro chromosome doubling was induced in octoploid (2n = 58) yacon using oryzalin and colchicine as mitotic spindle inhibitors. The resulting ploidy level was determined by chromosome counting and flow cytometry. The greatest proportions of hexadecaploid plants (1.6%) were obtained after 48 h of 25 lM oryzalin treatment. With the colchicine treatment, only 0.42% hexadecaploid plants were detected and their survival rate was significantly lower in comparison with the oryzalin treatment. In hexadecaploid yacon, significantly higher levels of saccharides were detected (FOS 13.9 g/100 g FM, fructose 4.6 g/100 g FM and glucose 2.1 g/100 g FM) compared to the octoploid control (FOS 5.3 g/100 g FM, fructose 2.9 g/100 g FM and glucose 1.0 g/100 g FM. These results indicate that in vitro treatment of nodal segments with oryzalin solution could be an effective procedure for chromosome doubling and the polyploidy breeding can help to increase the FOS content in the tuberous roots. In vitro chromosome doubling was induced in octoploid (2n = 58) yacon using oryzalin and colchicine as mitotic spindle inhibitors. The resulting ploidy level was determined by chromosome counting and flow cytometry. The greatest proportions of hexadecaploid plants (1.6%) were obtained after 48 h of 25 lM oryzalin treatment. With the colchicine treatment, only 0.42% hexadecaploid plants were detected and their survival rate was significantly lower in comparison with the oryzalin treatment. In hexadecaploid yacon, significantly higher levels of saccharides were detected (FOS 13.9 g/100 g FM, fructose 4.6 g/100 g FM and glucose 2.1 g/100 g FM) compared to the octoploid control (FOS 5.3 g/100 g FM, fructose 2.9 g/100 g FM and glucose 1.0 g/100 g FM. These results indicate that in vitro treatment of nodal segments with oryzalin solution could be an effective procedure for chromosome doubling and the polyploidy breeding can help to increase the FOS content in the tuberous roots. (en)
Title	In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) (en)
skos:prefLabel	In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) (en)
skos:notation	RIV/00027006:_____/09:00000767!RIV10-MZE-00027006
http://linked.open...avai/riv/aktivita	N S Z
http://linked.open...avai/riv/aktivity	N, S, Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Vyvadilová, Miroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	318920
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/09:00000767
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chromosome doubling; Colchicine; Flow cytometry; Hexadecaploid; Octoploid; Oryzalin; Smallanthus; Yacon (en)
http://linked.open.../riv/klicoveSlovo	Colchicine Yacon Oryzalin Flow cytometry Smallanthus Chromosome doubling Hexadecaploid Octoploid
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[69D773F15A8D]
http://linked.open...i/riv/nazevZdroje	Plant Cell, Tissue and Organ Culture
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	97
http://linked.open...iv/tvurceVysledku	Vyvadilová, Miroslava Viehmannová, I. Greplová, M. Bechyně, M. Cusimamani, F.
http://linked.open...ain/vavai/riv/wos	000263799100002
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0167-6857
number of pages	5 (xsd:int)
is http://linked.open...avai/riv/vysledek of	In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) In vitro induction of polyploidy in yacon (Smallanthus sonchifolius)

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