About: Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A pentaplex reverse-transcription polymerase chain reaction (Pentaplex RT-PCR) in a single tube was developed for the simultaneous detection of the pome fruit viruses: Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV). This is the first report of the simultaneous detection of all four viruses and host mRNA as an internal specific control. Pentaplex RT-PCR was applied successfully throughout the year, using different plant organs (leaves or dormant buds). The sensitivity of detection by monoplex-and pentaplex RT-PCR assays was comparable. Different combinations of mixed infections of viruses were identified in samples of infected apple and pear trees from different geographical regions. The pentaplex RT-PCR assay developed was sensitive, simple, rapid, and reliable for simultaneous detection of the four viruses in extracts of leaves or dormant buds. A pentaplex reverse-transcription polymerase chain reaction (Pentaplex RT-PCR) in a single tube was developed for the simultaneous detection of the pome fruit viruses: Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV). This is the first report of the simultaneous detection of all four viruses and host mRNA as an internal specific control. Pentaplex RT-PCR was applied successfully throughout the year, using different plant organs (leaves or dormant buds). The sensitivity of detection by monoplex-and pentaplex RT-PCR assays was comparable. Different combinations of mixed infections of viruses were identified in samples of infected apple and pear trees from different geographical regions. The pentaplex RT-PCR assay developed was sensitive, simple, rapid, and reliable for simultaneous detection of the four viruses in extracts of leaves or dormant buds. (en) Pentaplex RT-PCR byla vyvinuta pro detekci čtyř virů jádrovin: virus vrásčitosti kmene jabloně (ASPV), virus žlábkovitosti kmene jabloně (ASGV), Virus chlorotické skvrnitosti listů jabloně (ACLSV) a virus mozaiky jabloně. mRNA hostitele byla použita jako interní kontrola. (cs)
Title	Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR (en) Detekce a identifikace čtyř virů jádrovin pomocí pentaplex RT-PCR (cs)
skos:prefLabel	Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR (en) Detekce a identifikace čtyř virů jádrovin pomocí pentaplex RT-PCR (cs)
skos:notation	RIV/00027006:_____/06:2668!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany	124;129
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6046070901), Z(MZE0002700603)
http://linked.open...iv/cisloPeriodika	0
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Polák, Jaroslav Hassan, Mohamed
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	499455
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/06:2668
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Pentaplex RT-PCR; Woody indexing; ACLSV; ASPV; ApMV; ASGV; Pome fruit viruses (en)
http://linked.open.../riv/klicoveSlovo	ACLSV Pentaplex RT-PCR Pome fruit viruses Woody indexing ASGV ApMV ASPV
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[4DA786702BB4]
http://linked.open...i/riv/nazevZdroje	Journal of Virological Methods
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	133
http://linked.open...iv/tvurceVysledku	Polák, Jaroslav Hassan, Mohamed Myrta, A.
http://linked.open...n/vavai/riv/zamer	Setrvalé zemědělství, kvalita zemědělské produkce, krajinné a přírodní zdroje. Environment friendly systems of plant and stored product protection against harmful organisms aimed at quality and health safety plant products
issn	0166-0934
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR

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