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rdf:type
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Description
| - Renalní aktivita Akt kinázy, exprese a fosforylace mTOR , aktivita eNO syntázy byly studovány u obézních potkanů kmene Zucker a štíhlých kontrol. Aktivita AKT byla zvýšena u obézních potkanů paralelně s hyperizulinémií. Ve svalech rozdíly v AKT nebyly zjištěny. U obézních potkanů byla zvýšena fosforylace mTOR. Akutní inhibice PI3K wortmaninem snížila renální aktivitu Akt a mTOR u obézních ale nikoliv u štíhlých potkanů. (cs)
- Renal cortical Akt was determined in obese Zucker rats (ZO), a model of DM2, and lean controls (ZL). We also studied expression and phosphorylation of the mammalian target of rapamycin (mTOR) and endothelial NO synthase (eNOS), molecules downstream of Akt in the insulin signaling cascade, and documented modulators of renal injury. Akt activity was measured by a kinase assay with GSK-3 as a substrate. Expression of phosphorylated (active) and total proteins was measured by immunoblotting and immunohistochemistry. Renal Akt activity was increased in ZO as compared to ZL rats, in parallel with progressive hyperinsulinemia. No differences in Akt were observed in the skeletal muscle. Corresponding to increases in Akt activity, ZO rats demonstrated enhanced phosphorylation of renal mTOR. Acute PI3K inhibition with wortmannin (100 mug/kg) attenuated renal Akt and mTOR activities in ZO, but not in ZL rats.
- Renal cortical Akt was determined in obese Zucker rats (ZO), a model of DM2, and lean controls (ZL). We also studied expression and phosphorylation of the mammalian target of rapamycin (mTOR) and endothelial NO synthase (eNOS), molecules downstream of Akt in the insulin signaling cascade, and documented modulators of renal injury. Akt activity was measured by a kinase assay with GSK-3 as a substrate. Expression of phosphorylated (active) and total proteins was measured by immunoblotting and immunohistochemistry. Renal Akt activity was increased in ZO as compared to ZL rats, in parallel with progressive hyperinsulinemia. No differences in Akt were observed in the skeletal muscle. Corresponding to increases in Akt activity, ZO rats demonstrated enhanced phosphorylation of renal mTOR. Acute PI3K inhibition with wortmannin (100 mug/kg) attenuated renal Akt and mTOR activities in ZO, but not in ZL rats. (en)
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Title
| - Renal activity of Akt kinase in obese Zucker rats
- Renal activity of Akt kinase in obese Zucker rats (en)
- Renální aktivita Akt kinázy u obézních potkanů Zucker. (cs)
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skos:prefLabel
| - Renal activity of Akt kinase in obese Zucker rats
- Renal activity of Akt kinase in obese Zucker rats (en)
- Renální aktivita Akt kinázy u obézních potkanů Zucker. (cs)
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skos:notation
| - RIV/00023001:_____/08:00001897!RIV09-MZ0-00023001
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00023001:_____/08:00001897
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - akt kinase; Zucker rats; NO synthase; diabetes; insulin (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Experimental biology and medicine (Maywood)
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Komers, Radko
- Pelikánová, Terezie
- Kazdová, Ludmila
- Ždychová, Jana
- Anderson, S.
- Linsley, JN
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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