About: 34-21 Production of clones embryo     Goto   Sponge   Distinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

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Description
  • We will be concetrated on pig oocytes maturation in vitro, their artificial activation and their subsequent invasive enucleation by mikromanipulation.Development of an effective activation and invasive enucleation protocols is of great importance for studying oocytes competence and embryo cloning. We would like to also try the noninvasive chemical enucleation, because this method of enucleation hasn´t been developed in pigs yet. This novel enucleation technique could greatly facilitate the research in pig cloning experiments. (en)
  • Althrough the birth of the first lamb produced by nuclear transfer of differentiated cell into the enucleated metaphase II oocyte has been reported, the success of current cloning procedures is still extremely low for unknown reasons. We do not know if the differentiation of karyoplasts and our inability to reprogramme them is the main reason why the development of clones is so poor. Another factor, which may well be involved is the synchronization of cell cycles between karyoplasts and cytoplasts. The purpose of our cooperation project is the detailed study of nucleo-cytoplasmic interactions in reconstructed embryo and the effect of different combinations upon nuclear reprogramming and futher development.
Title
  • 34-21 Production of clones embryo (en)
  • Produkce klonovaných jedinců
skos:notation
  • ME 289
http://linked.open...avai/cep/aktivita
http://linked.open...kovaStatniPodpora
http://linked.open...ep/celkoveNaklady
http://linked.open...ep/duvernostUdaju
http://linked.open.../cep/fazeProjektu
http://linked.open...ai/cep/hlavniObor
http://linked.open...hodnoceniProjektu
http://linked.open.../cep/klicovaSlova
  • pig,oocytes,maturation,activation,enucleation,recipient cytoplasm (en)
http://linked.open...ep/partnetrHlavni
http://linked.open...inujicichPrijemcu
http://linked.open...cep/pocetPrijemcu
http://linked.open...ocetSpoluPrijemcu
http://linked.open.../pocetVysledkuRIV
http://linked.open...enychVysledkuVRIV
http://linked.open...iciPoslednihoRoku
http://linked.open...atUdajeProjZameru
http://linked.open...usZobrazovaneFaze
http://linked.open...ai/cep/typPojektu
http://linked.open...jektu+dodavatelem
  • Závěrečná zpráva (cs)
http://linked.open...tniCyklusProjektu
http://linked.open.../cep/klicoveSlovo
  • activation
  • pig
  • oocytes
  • maturation
  • enucleation
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