A)Identifikace genu Fw, zodpovídajícího za úplnou rezistenci k fuzáriovému vadnutí (Fusarium oxysporum f.sp. pisi - rase 1). B)Ověření hypotézy, že FRR2 lokus rezistence ke kořenové fuzáriové spále hrachu (Fusarium solani), odpovídá genům DRR206, pI39. (cs)
Direct markers are the next evolution in crop improvement strategies. Modern plant breeding programs employ DNA markers linked to the trait of interest and PCR technologies to quickly identify lines to improve efficiencies in releasing agronomically superior cultivars for pea crop production. With the advent of cloning the genes/alleles controlling economic traits like quality and disease resistances, traits can be ed directly using PCR technology. We proposed to fine map the loci we have identified for resistance to Fusarium root rot and Fusarium wilt, using bulk segregant analysis of recombinants flanking important resistance loci for each disease. For fine mapping, candidate genes will be utilized from sophisticated plant-microbe molecular interaction studies. Validation of the candidate gene(s) effect (s) will be by association mapping in a germplasm population previously phenotyped for reaction to Fusarium root rot and Fusarium wilt. (en)