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rdf:type
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Description
| - While γ-tubulin 1 is ubiquitous, γ-tubulin 2 is known to be not able to substitute for γ-tubulin 1 deficiency in blastocysts. This research article presents our contribution to deciphering of γ-tubulin 2 role. We have found that exogenous γ-tubulin 2 is targeted to centrosomes and interacts with γ-tubulin complex proteins 2 and 4. Depletion of γ-tubulin 1 by RNAi in U2Os cells causes impaired microtubule nucleation and metaphase arrest. Wild-type phenotype in γ-tubulin 1-depleted cells is restored by expression of exogenous mouse and human γ-tubulin 2. Using RT-qPCR and 2D-PAGE, we have found that Tubg2 expression is dramatically reduced in mouse blastocysts. This indicates that γ-tubulin 2 cannot rescue γ-tubulin 1 deficiency in knock-out blastocysts. The obtained data suggest that γ-tubulin 2 is able to nucleate microtubules and subtitute for γ-tubulin 1. We propose that mammalian γ-tubulins are functionally redundant with respect to the nucleation activity of microtubules.
- While γ-tubulin 1 is ubiquitous, γ-tubulin 2 is known to be not able to substitute for γ-tubulin 1 deficiency in blastocysts. This research article presents our contribution to deciphering of γ-tubulin 2 role. We have found that exogenous γ-tubulin 2 is targeted to centrosomes and interacts with γ-tubulin complex proteins 2 and 4. Depletion of γ-tubulin 1 by RNAi in U2Os cells causes impaired microtubule nucleation and metaphase arrest. Wild-type phenotype in γ-tubulin 1-depleted cells is restored by expression of exogenous mouse and human γ-tubulin 2. Using RT-qPCR and 2D-PAGE, we have found that Tubg2 expression is dramatically reduced in mouse blastocysts. This indicates that γ-tubulin 2 cannot rescue γ-tubulin 1 deficiency in knock-out blastocysts. The obtained data suggest that γ-tubulin 2 is able to nucleate microtubules and subtitute for γ-tubulin 1. We propose that mammalian γ-tubulins are functionally redundant with respect to the nucleation activity of microtubules. (en)
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Title
| - γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis
- γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis (en)
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skos:prefLabel
| - γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis
- γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis (en)
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skos:notation
| - RIV/68378050:_____/12:00375928!RIV13-GA0-68378050
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(1M0506), P(GA204/09/1777), P(GAP302/10/1701), P(GD204/09/H084), P(KAN200520701), P(LC545), Z(AV0Z50520514)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/12:00375928
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - γ-tubulin 2; nucleation; embryogenesis (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Dráber, Pavel
- Dráberová, Eduarda
- Sulimenko, Tetyana
- Vinopal, Stanislav
- Flemr, Matyáš
- Sulimenko, Vadym
- Vosecká, Věra
- Černohorská, Markéta
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://bibframe.org/vocab/doi
| - 10.1371/journal.pone.0029919
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