About: Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Protein tepelného šoku 90 (Hsp90) je molekulární chaperon hojně se vyskytující v eukaryotických buňkách. Jeho přesná úloha však ještě nebyla zcela vyjasněna. Pomocí testu na vazbu železa a analýzy hmotnostní spektrometrií jsme v preparátech membránových proteinů z lidských buněk HeLa identifikovali lidský Hsp90 jako protein vázající železo. Analýza Westernova přenosu a konfokální mikroskopie potvrdila, že část buněčného Hsp90 je spojena s plasmatickou membránou, ale není zřejmě exprimován na buněčném povrchu. Test na vazbu železa s purifikovaným lidským Hsp90 potvrdil, že Hsp90 váže železo. Vyvozujeme z toho, že Hsp90 je protein vázající železo spojený s plasmatickou membránou (cs) Heat shock protein 90 (Hsp90) is a molecular chaperone abundant in eukaryotic cells. However, its exact role is not completely understood yet. Employing an iron-binding assay and mass spectrometric analysis, we have identified human Hsp90 as an iron-binding protein in membrane protein preparations of human HeLa cells. Western blot analysis and confocal microscopy confirmed that a portion of cellular Hsp90 is associated with the plasma membrane, but it does not seem to be expressed on the cell surface. The iron-binding assay with purified human Hsp90 confirmed iron binding by Hsp90. Thus we suggest that Hsp90 is an iron-binding protein ssociated with the plasma membrane Heat shock protein 90 (Hsp90) is a molecular chaperone abundant in eukaryotic cells. However, its exact role is not completely understood yet. Employing an iron-binding assay and mass spectrometric analysis, we have identified human Hsp90 as an iron-binding protein in membrane protein preparations of human HeLa cells. Western blot analysis and confocal microscopy confirmed that a portion of cellular Hsp90 is associated with the plasma membrane, but it does not seem to be expressed on the cell surface. The iron-binding assay with purified human Hsp90 confirmed iron binding by Hsp90. Thus we suggest that Hsp90 is an iron-binding protein ssociated with the plasma membrane (en)
Title	Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells (en) Protein tepelného šoku 90 jako protein vázající železo spojený s plasmatickou membránou buněk HeLa (cs)
skos:prefLabel	Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells Protein 90 Recognized as an Iron-Binding Protein Associated with the Plasma Membrane of HeLa Cells (en) Protein tepelného šoku 90 jako protein vázající železo spojený s plasmatickou membránou buněk HeLa (cs)
skos:notation	RIV/68378050:_____/04:00108974!RIV/2005/AV0/A23005/N
http://linked.open.../vavai/riv/strany	41;46
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA301/01/0041), P(IAA5052702)
http://linked.open...iv/cisloPeriodika	14
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Kovář, Jan Koc, Michal Truksa, Jaroslav Štýbrová, Hana Kriegerbecková, Karin Ehrlichová, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	582870
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/04:00108974
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	heat shock protein 90; iron-binding protein; plasma membrane (en)
http://linked.open.../riv/klicoveSlovo	plasma membrane heat shock protein 90 iron-binding protein
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[79CC642EBB31]
http://linked.open...i/riv/nazevZdroje	Cellular Physiology and Biochemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Mechanisms of resistance to induction of apoptosis in tumor cells Membrane transport of non-transferrin iron in mammalian cells
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	1-2
http://linked.open...iv/tvurceVysledku	Koc, Michal Kovář, Jan Novák, J. Truksa, Jaroslav Ehrlichová, Marie Kriegerbecková, Karin Goldenberg, H. Štýbrová, Hana Scheiber-Mojdehkar, B.
issn	1015-8987
number of pages	6 (xsd:int)

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