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rdf:type
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Description
| - We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented.
- We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented. (en)
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Title
| - Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection.
- Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection. (en)
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skos:prefLabel
| - Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection.
- Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection. (en)
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skos:notation
| - RIV/68081715:_____/02:25033002!RIV/2004/AV0/A25004/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(IAA4031901), Z(AV0Z4031919), Z(MSM 263100020)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68081715:_____/02:25033002
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - capillary isoelectric focusing; fluorescence detection; pI standards (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...nichUcastnikuAkce
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Šlais, Karel
- Horká, Marie
- Friedl, Z.
- Nováčková, J.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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