About: Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection.     Goto   Sponge   Distinct   Permalink

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Description
  • We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented.
  • We prepared a series of low-molecular-mass fluorescent ampholytes with narrow pI range. These fluorescein-based ampholytes are detection compatible with argon laser-induced fluorescence (LIF) detection. The selected properties, important for their routine use as fluorescent pI markers, were examined. The pI values of new fluorescein-based pI markers were determined by capillary isoelectric focusing (CIEF) using currently available low-molecular-mass pI markers for CIEF with photometric detection. The examples of CIEF with fluorometric detection of new compounds together with fluorescein isothiocyanate (FITC) derivatized proteins are presented. (en)
Title
  • Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection.
  • Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection. (en)
skos:prefLabel
  • Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection.
  • Fluorescein-based pImarkers for capillary isoelectric focusing with laser-induced fluorescence detection. (en)
skos:notation
  • RIV/68081715:_____/02:25033002!RIV/2004/AV0/A25004/N
http://linked.open.../vavai/riv/strany
  • 1682;1688
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(IAA4031901), Z(AV0Z4031919), Z(MSM 263100020)
http://linked.open...iv/cisloPeriodika
  • 11
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 646358
http://linked.open...ai/riv/idVysledku
  • RIV/68081715:_____/02:25033002
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • capillary isoelectric focusing; fluorescence detection; pI standards (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [DC0683AD275D]
http://linked.open...i/riv/nazevZdroje
  • Electrophoresis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 23
http://linked.open...iv/tvurceVysledku
  • Šlais, Karel
  • Horká, Marie
  • Friedl, Z.
  • Nováčková, J.
http://linked.open...n/vavai/riv/zamer
issn
  • 0173-0835
number of pages
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