About: Charge heterogeneity of commercial, red-shifted recombinant green fluorescent protein, revealed by capillary zone electrophoresis under nondenaturing conditions.     Goto   Sponge   Distinct   Permalink

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Description
  • The demonstration in electrophoresis of charge isomerism of rEGFP nonderivatized with SDS presents an illustration for the fact that apparent homogeneity of a protein in SDS-PAGE only refers to the molecular weight of the gene product and is blind to posttranslational modifications or other sources of isomerism. In this respect, CZE combined with the Ferguson plot analysis of recombinant proteins, compared to conventional SDS-PAGE.
  • The demonstration in electrophoresis of charge isomerism of rEGFP nonderivatized with SDS presents an illustration for the fact that apparent homogeneity of a protein in SDS-PAGE only refers to the molecular weight of the gene product and is blind to posttranslational modifications or other sources of isomerism. In this respect, CZE combined with the Ferguson plot analysis of recombinant proteins, compared to conventional SDS-PAGE. (en)
Title
  • Charge heterogeneity of commercial, red-shifted recombinant green fluorescent protein, revealed by capillary zone electrophoresis under nondenaturing conditions.
  • Charge heterogeneity of commercial, red-shifted recombinant green fluorescent protein, revealed by capillary zone electrophoresis under nondenaturing conditions. (en)
skos:prefLabel
  • Charge heterogeneity of commercial, red-shifted recombinant green fluorescent protein, revealed by capillary zone electrophoresis under nondenaturing conditions.
  • Charge heterogeneity of commercial, red-shifted recombinant green fluorescent protein, revealed by capillary zone electrophoresis under nondenaturing conditions. (en)
skos:notation
  • RIV/68081715:_____/00:25200075!RIV/2003/AV0/A25003/N
http://linked.open.../vavai/riv/strany
  • 146;148
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  • Z(AV0Z4031919)
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  • 706638
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  • RIV/68081715:_____/00:25200075
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  • N/A (en)
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  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [0811002A72D6]
http://linked.open...i/riv/nazevZdroje
  • Analytical Biochemistry
http://linked.open...in/vavai/riv/obor
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http://linked.open...v/svazekPeriodika
  • 274
http://linked.open...iv/tvurceVysledku
  • Šťastná, Miroslava
  • Chrambach, A.
  • Radko, S. P.
  • Buzas, S.
  • Kingsley, D.
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-2697
number of pages
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