About: Advanced Microscopy Techniques Used for Comparison of UVA- and gamma-Irradiation-Induced DNA Damage in the Cell Nucleus and Nucleolus

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About: Advanced Microscopy Techniques Used for Comparison of UVA- and gamma-Irradiation-Induced DNA Damage in the Cell Nucleus and Nucleolus Goto Sponge Distinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Every day, genomes are affected by genotoxic factors that create multiple DNA lesions. Several DNA repair systems have evolved to counteract the deleterious effects of DNA damage. These systems include a set of DNA repair mechanisms, damage tolerance processes, and activation of cell-cycle checkpoints. This study describes selected confocal microscopy techniques that investigate DNA damage-related nuclear events after UVA- and gamma-irradiation and compare the DNA damage response (DDR) induced by the two experimental approaches. In both cases, we observed induction of the nucleotide excision repair (NER) pathway and formation of localized double-strand breaks (DSBs). This was confirmed by analysis of cyclobutane pyrimidine dimers (CPDs) in the DNA lesions and by increased levels of gamma H2AX and 53BP1 proteins in the irradiated genome. DNA damage by UVA-lasers was potentiated by either BrdU or Hoechst 33342 pre-sensitization and compared to non-photosensitized cells. Every day, genomes are affected by genotoxic factors that create multiple DNA lesions. Several DNA repair systems have evolved to counteract the deleterious effects of DNA damage. These systems include a set of DNA repair mechanisms, damage tolerance processes, and activation of cell-cycle checkpoints. This study describes selected confocal microscopy techniques that investigate DNA damage-related nuclear events after UVA- and gamma-irradiation and compare the DNA damage response (DDR) induced by the two experimental approaches. In both cases, we observed induction of the nucleotide excision repair (NER) pathway and formation of localized double-strand breaks (DSBs). This was confirmed by analysis of cyclobutane pyrimidine dimers (CPDs) in the DNA lesions and by increased levels of gamma H2AX and 53BP1 proteins in the irradiated genome. DNA damage by UVA-lasers was potentiated by either BrdU or Hoechst 33342 pre-sensitization and compared to non-photosensitized cells. (en)
Title	Advanced Microscopy Techniques Used for Comparison of UVA- and gamma-Irradiation-Induced DNA Damage in the Cell Nucleus and Nucleolus Advanced Microscopy Techniques Used for Comparison of UVA- and gamma-Irradiation-Induced DNA Damage in the Cell Nucleus and Nucleolus (en)
skos:prefLabel	Advanced Microscopy Techniques Used for Comparison of UVA- and gamma-Irradiation-Induced DNA Damage in the Cell Nucleus and Nucleolus Advanced Microscopy Techniques Used for Comparison of UVA- and gamma-Irradiation-Induced DNA Damage in the Cell Nucleus and Nucleolus (en)
skos:notation	RIV/68081707:_____/14:00435966!RIV15-GA0-68081707
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(EE2.3.30.0030), P(GBP302/12/G157)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Legartová, Soňa Stixová, Lenka Bártová, Eva Kozubek, Stanislav Sehnalová, Petra Hrušková, Tereza
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	1731
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/14:00435966
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	UVA; gamma-irradiation; DNA damage response (en)
http://linked.open.../riv/klicoveSlovo	gamma-irradiation UVA DNA damage response
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[B90C5E1FE09C]
http://linked.open...i/riv/nazevZdroje	Folia Biologica
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions Development of human resources in the field of cell biology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Bártová, Eva Kozubek, Stanislav Legartová, Soňa Stixová, Lenka Svidenská, S. Hrušková, Tereza Sehnalová, Petra
http://linked.open...ain/vavai/riv/wos	000343275800013
issn	0015-5500
number of pages	9 (xsd:int)

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