About: GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	GFP-like 3,5-difluoro-4-hydroxybenzylideneimidazolinone and 3,5-bis(methoxy)-4-hydroxy-benzylideneimidazolinone labels were attached to dCTP through propargyl linker and the resulting labeled nucleotides were used for a facile enzymatic synthesis of oligonucleotide or DNA probes by polymerase-catalyzed primer extension. The labeled DNA probes exerted low fluorescence which was increased 2-3.2 times upon binding of a protein. The FBI label was also used for a time-resolved experiment monitoring a single-nucleotide incorporation followed by primer extension by Vent(exo-) polymerase. GFP-like 3,5-difluoro-4-hydroxybenzylideneimidazolinone and 3,5-bis(methoxy)-4-hydroxy-benzylideneimidazolinone labels were attached to dCTP through propargyl linker and the resulting labeled nucleotides were used for a facile enzymatic synthesis of oligonucleotide or DNA probes by polymerase-catalyzed primer extension. The labeled DNA probes exerted low fluorescence which was increased 2-3.2 times upon binding of a protein. The FBI label was also used for a time-resolved experiment monitoring a single-nucleotide incorporation followed by primer extension by Vent(exo-) polymerase. (en)
Title	GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions (en)
skos:prefLabel	GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions (en)
skos:notation	RIV/68081707:_____/12:00382270!RIV13-AV0-68081707
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA203/09/0317), P(GBP206/12/G151)
http://linked.open...iv/cisloPeriodika	18
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Fojta, Miroslav Orság, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	138142
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/12:00382270
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA; oligonucleotides; polymerase; nucleotides; fluorescent labeling (en)
http://linked.open.../riv/klicoveSlovo	polymerase DNA fluorescent labeling nucleotides oligonucleotides
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[6EC053D0B686]
http://linked.open...i/riv/nazevZdroje	Journal of Organic Chemistry
http://linked.open...in/vavai/riv/obor	CC
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of novel approaches to bioanalysis and molecular diagnostics Construction of novel functional nucleic acids for applications in chemical biology, catalysis and self assembly.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	77
http://linked.open...iv/tvurceVysledku	Fojta, Miroslav Hocek, Michal Pohl, Radek Orság, Petr Ménová, Petra Riedl, Jan
http://linked.open...ain/vavai/riv/wos	000308957700049
issn	0022-3263
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/jo301684b

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