About: A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Bacterial populations display high heterogeneity in viability and physiological activity at the single-cell level, especially under stressful conditions . We demonstrate a novel staining protocol for multiparameter assessment of individual cells in physiologically heterogenous populations of canobacteria.The protocol employs fluorescent probes , i.e., redox dye 5-cyano-2,3-diotyl tetrazolium chloride, SYTOX Green, and DNA-specific fluorchrome 4,6-diamidino-2phenylindole, combined with microscopy image analysis. Our method allows simultaneous estimates of cellular respiration activity, membrane and nucleoid integrity, and allows the detection of photosynthetic pigments fluorescence along with morphological observations. We adjusted laboratory and natural populations of the genus phormidium. Cassification of cells in cyanobacterial populations was into four categories:activite and intact; injured but activite; metabolically inactivite but intact; inactive and injured or dead. Bacterial populations display high heterogeneity in viability and physiological activity at the single-cell level, especially under stressful conditions . We demonstrate a novel staining protocol for multiparameter assessment of individual cells in physiologically heterogenous populations of canobacteria.The protocol employs fluorescent probes , i.e., redox dye 5-cyano-2,3-diotyl tetrazolium chloride, SYTOX Green, and DNA-specific fluorchrome 4,6-diamidino-2phenylindole, combined with microscopy image analysis. Our method allows simultaneous estimates of cellular respiration activity, membrane and nucleoid integrity, and allows the detection of photosynthetic pigments fluorescence along with morphological observations. We adjusted laboratory and natural populations of the genus phormidium. Cassification of cells in cyanobacterial populations was into four categories:activite and intact; injured but activite; metabolically inactivite but intact; inactive and injured or dead. (en)
Title	A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes (en)
skos:prefLabel	A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogenity in Phormidium Populations( Cyanobacteria) Employing Fluorescent Dyes (en)
skos:notation	RIV/67985939:_____/13:00393243!RIV14-AV0-67985939
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(LA341), P(ME 934)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Elster, Josef
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Botanický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	58918
http://linked.open...ai/riv/idVysledku	RIV/67985939:_____/13:00393243
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cyanobacteria; physiological activity; fluorescent dyes (en)
http://linked.open.../riv/klicoveSlovo	fluorescent dyes physiological activity Cyanobacteria
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[3171A3D25775]
http://linked.open...i/riv/nazevZdroje	PLoS ONE
http://linked.open...in/vavai/riv/obor	EH
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biological and climat diverzity of central part of Svalbard Arctic archipelago http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20934
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Elster, Josef Tashyreva, D. Billi, D.
http://linked.open...ain/vavai/riv/wos	000315184200014
issn	1932-6203
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0055283

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