About: Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point

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About: Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point Goto Sponge Distinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Several methods of molecular analysis of microbial diversity, including terminal restriction fragment length polymorphism (T-RFLP) analysis are based on measurement of the DNA fragment length. Significant variation between sequence-determined and measured length of restriction fragments (drift) has been observed, which can affect the efficiency of the identification of microorganisms in the analyzed communities. In this study, principal component analysis and multiple regression analysis revealed that secondary structure melting point and G+C nucleotide content, besides the fragment length, contribute to the variation observed, whereas the contribution of purine Several methods of molecular analysis of microbial diversity, including terminal restriction fragment length polymorphism (T-RFLP) analysis are based on measurement of the DNA fragment length. Significant variation between sequence-determined and measured length of restriction fragments (drift) has been observed, which can affect the efficiency of the identification of microorganisms in the analyzed communities. In this study, principal component analysis and multiple regression analysis revealed that secondary structure melting point and G+C nucleotide content, besides the fragment length, contribute to the variation observed, whereas the contribution of purine (en)
Title	Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point (en)
skos:prefLabel	Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point (en)
skos:notation	RIV/67985939:_____/10:00347130!RIV11-MSM-67985939
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0571), Z(AV0Z50200510), Z(AV0Z60050516)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Bukovská, Petra Sýkorová, Zuzana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Botanický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	292384
http://linked.open...ai/riv/idVysledku	RIV/67985939:_____/10:00347130
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	T-RFLP; drift; DNA fragment (en)
http://linked.open.../riv/klicoveSlovo	DNA fragment drift T-RFLP
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[02F739C14451]
http://linked.open...i/riv/nazevZdroje	Journal of Microbiological Methods
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Bioindication and revitalization of toxic and antropogenic substrates and water sources: application of cyano bacteria, algae, soil bacteria and symbiotic fungi
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	82
http://linked.open...iv/tvurceVysledku	Sýkorová, Zuzana Gryndler, Milan Hršelová, Hana Jelínková, Markéta Bukovská, Petra
http://linked.open...ain/vavai/riv/wos	000282113000005
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Struktura, funkce a evoluce biodiversity fotoautotrofních organismů a hub: původ a příčiny jejich proměnlivosti, dynamika populací, společenstev a ekosystémů; využití vybraných výsledků k rozvoji Průhonického parku
issn	0167-7012
number of pages	6 (xsd:int)

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