About: Molecular mechanism of pregnenolone sulfate action at NR1/NR2B receptors

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Whole-cell patch-clamp recording from human embryonic kidney 293 cells expessing NR1-1a/NR2B receptors revealed that neurosteroid pregnenolone sulfate (PS) (300mm), when applied to resting NMDA receptors, potentiates the amplitude of subsequent responses to 1 mM glutamate fivefold and slows their deactivation twofold. The same concetration of PS, when applied during NMDA receptor activation by 1nM glutamate, has only a small effect. These results suggest that the PS is a modulator of NMDA receptor Po, the effectiveness of which is lowered by glutamate binding Whole-cell patch-clamp recording from human embryonic kidney 293 cells expessing NR1-1a/NR2B receptors revealed that neurosteroid pregnenolone sulfate (PS) (300mm), when applied to resting NMDA receptors, potentiates the amplitude of subsequent responses to 1 mM glutamate fivefold and slows their deactivation twofold. The same concetration of PS, when applied during NMDA receptor activation by 1nM glutamate, has only a small effect. These results suggest that the PS is a modulator of NMDA receptor Po, the effectiveness of which is lowered by glutamate binding (en) Rekombinantní NR1-1a/NR2B receptory byly exprimovány v lidských embryonálních ledvinných (HEK293) buňkách, ze kterých byly snímány odpovědi pomocí metody patch-clamp. Zjistili jsme, že pokud preaplikujeme pregnenolon sulfát (PS; 300 mM) na agonisty neaktivované NR1-1a/NR2B receptory, dochází k pětinásobné potenciaci odpovědí těchto receptorů a zároveň ke dvojnásobnému zpomalení doby deaktivace. Stejná koncentrace PS aplikovaná na NR1-1a/NR2B receptory aktivované předchozí aplikací glutamátu potencuje odpovědi těchto receptorů minimálně. Naše výsledky ukazují, že PS je modulátor pravděpodobnosti otevření NR1-1a/NR2B receptorů, jehož účinnost je snížena vazbou glutamátu (cs)
Title	Molecular mechanism of pregnenolone sulfate action at NR1/NR2B receptors Molekulární mechanizmus účinku pregnenolon sulfátu na NR1/NR2B receptorech (cs) Molecular mechanism of pregnenolone sulfate action at NR1/NR2B receptors (en)
skos:prefLabel	Molecular mechanism of pregnenolone sulfate action at NR1/NR2B receptors Molekulární mechanizmus účinku pregnenolon sulfátu na NR1/NR2B receptorech (cs) Molecular mechanism of pregnenolone sulfate action at NR1/NR2B receptors (en)
skos:notation	RIV/67985823:_____/04:00102547!RIV/2005/AV0/A20005/N
http://linked.open.../vavai/riv/strany	10318;10325
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA309/04/1537), P(IAA4055305), P(LN00B122), Z(AV0Z4055905), Z(AV0Z5011922)
http://linked.open...iv/cisloPeriodika	46
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Vyklický ml., Ladislav Vlček, Kamil Horák, Martin Petrovič, Miloš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	574389
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/04:00102547
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	neurosteroids;NMDA receptor;glutamate (en)
http://linked.open.../riv/klicoveSlovo	NMDA receptor glutamate neurosteroids
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1DD24B4D01E1]
http://linked.open...i/riv/nazevZdroje	Journal of Neuroscience
http://linked.open...in/vavai/riv/obor	FH
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of the effects of neurosteroids on ionotropic glutamate receptors and synaptic transmission Neuropsychiatric Research Center Synthetic analogues of neurosteroids with activity on membrane receptors
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	24
http://linked.open...iv/tvurceVysledku	Chodounská, Hana Horák, Martin Vyklický ml., Ladislav Vlček, Kamil Petrovič, Miloš
http://linked.open...n/vavai/riv/zamer	Investigation of normal and pathological physiology of animals - the clarification of the physiological mechanisms operating in humans in health and disease Chemical principles of selected biological phenomena in medicine and ecology
issn	0270-6474
number of pages	8 (xsd:int)

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