Attributes | Values |
---|
rdf:type
| |
Description
| - Low-density membrane fragments (domains) were separated from the bulk plasma membranes of HEK293 cells expressing DOP receptor-Gi1alpha fusion protein by drastic homogenization and flotation on sucrose density gradient. Low-density domains exhibited four times higher agonist-stimulated GTPase activity and GTPgammaS binding than the bulk plasma membranes (when corrected for expression levels of the receptor). RGS1 enhanced further the G protein functional activity but did not remove the difference between domain-bound and plasma membrane pools of G protein. By contrast, detergent-extraction of membrane domains diminished functional coupling between G proteinůcoupled receptor and corresponding G protein.
- Low-density membrane fragments (domains) were separated from the bulk plasma membranes of HEK293 cells expressing DOP receptor-Gi1alpha fusion protein by drastic homogenization and flotation on sucrose density gradient. Low-density domains exhibited four times higher agonist-stimulated GTPase activity and GTPgammaS binding than the bulk plasma membranes (when corrected for expression levels of the receptor). RGS1 enhanced further the G protein functional activity but did not remove the difference between domain-bound and plasma membrane pools of G protein. By contrast, detergent-extraction of membrane domains diminished functional coupling between G proteinůcoupled receptor and corresponding G protein. (en)
|
Title
| - Delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains.
- Delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains. (en)
|
skos:prefLabel
| - Delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains.
- Delta-Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains. (en)
|
skos:notation
| - RIV/67985823:_____/03:20030014!RIV/2004/MSM/A20004/N
|
http://linked.open.../vavai/riv/strany
| |
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(GA309/01/0255), P(LN00A026), Z(AV0Z5011922), Z(MSM 113100003)
|
http://linked.open...iv/cisloPeriodika
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/67985823:_____/03:20030014
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - membrane domains; GTPase activity; G protein coupling (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
|
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...i/riv/nazevZdroje
| - Journal of Neurochemistry
|
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...ocetUcastnikuAkce
| |
http://linked.open...nichUcastnikuAkce
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...v/svazekPeriodika
| |
http://linked.open...iv/tvurceVysledku
| - Novotný, Jiří
- Svoboda, Petr
- Hejnová, Lucie
- Bouřová, Lenka
- Moravcová, Zuzana
- Milligan, G.
- Koštrnová, Alexandra
- Moon, H. E.
|
http://linked.open...n/vavai/riv/zamer
| |
issn
| |
number of pages
| |