About: Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This paper describes principles of a proper dilution of samples used for the determination of antioxidant capacity by means of a photochemiluminiscence method using the instrument Photochem (Analytik Jena AG). The authors used the method ACL (Lipid-soluble Antioxidant Capacity), which is one of two methods enabling to measure in this instrument. It was demonstrated that values measured by the photochemiluminiscence method ACL were infl uenced by the degree of sample dilution. When studying effects of dilution of samples of wine and rutin, it was demonstrated that there is a nonlinear correlation between the degree of dilution and measured values of antioxidant capacity. At low molar amount of substance (i.e. at 0.5 and 1.0 nmol), the measured value of rutin inhibition was higher than the same that molar amount of trolox. At higher molar amounts (i.e. 3; 4 and 5 nmol), the inhibition value of trolox was higher. This dependent change can be explained by means of a diff erent eff ectiveness of antioxidants and a diff erent stability of their products with radicals. This paper describes principles of a proper dilution of samples used for the determination of antioxidant capacity by means of a photochemiluminiscence method using the instrument Photochem (Analytik Jena AG). The authors used the method ACL (Lipid-soluble Antioxidant Capacity), which is one of two methods enabling to measure in this instrument. It was demonstrated that values measured by the photochemiluminiscence method ACL were infl uenced by the degree of sample dilution. When studying effects of dilution of samples of wine and rutin, it was demonstrated that there is a nonlinear correlation between the degree of dilution and measured values of antioxidant capacity. At low molar amount of substance (i.e. at 0.5 and 1.0 nmol), the measured value of rutin inhibition was higher than the same that molar amount of trolox. At higher molar amounts (i.e. 3; 4 and 5 nmol), the inhibition value of trolox was higher. This dependent change can be explained by means of a diff erent eff ectiveness of antioxidants and a diff erent stability of their products with radicals. (en)
Title	Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method (en)
skos:prefLabel	Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method Effect of sample dilution on estimated values of antioxidant capacity by photochemiluminiscence method (en)
skos:notation	RIV/62156489:43510/12:00193466!RIV13-MZE-43510___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QI91B094)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Balík, Josef Híc, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Zahradnická fakulta (Lednice)
http://linked.open...dnocenehoVysledku	133234
http://linked.open...ai/riv/idVysledku	RIV/62156489:43510/12:00193466
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antioxidant capacity; photochem; sample dilution; photochemiluminiscence (en)
http://linked.open.../riv/klicoveSlovo	antioxidant capacity photochem photochemiluminiscence sample dilution
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[1FECFDCD7F76]
http://linked.open...i/riv/nazevZdroje	Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
http://linked.open...in/vavai/riv/obor	GC
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Grape juices from given sort of vine with health benefit
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Balík, Josef Híc, Pavel
issn	1211-8516
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	43510

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