Description
| - The aim of this study was utilize microsatellites for routine parentage testing and identification in breed cattle discrimination. The analysis was performed in three beef cattle (Aberdeen Angus n= 93, Hereford n= 125, Charolais n = 101) and one dairy cattle breed (Czech Fleckvieh n = 46). In total, 365 animals were genotyped with using Finnish Bovine Genotypes Panel 3.1 for 18 microsatellite markers recommended by both ISAG for parentage testing and FAO for genetic studies of domestic animals (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824, BM1818, and SPS113, RM067, CSRM60, MGTG4B, CSSM66, ILSTS006, respectively). The STRUCTURE (version 2.2) program based on a Bayesian clustering method was used to obtain population structure. The software detected four clusters corresponding to the four breeds without using prior information. The genetic markers used in study were suitable for good clustering ability by evidencing good discrimination power. Differentiation among individuals of each breed characterized by Fst values range from the lowest 0.091 in Aberdeen Angus, 0.099 in Czech Fleckvieh, 0.122 in Charolais, to highest 0.146 in Hereford cattle population. Average distances (expected heterozygosity) between individuals in same cluster were for Czech Fleckvieh 0.684, Charolais 0.702, Aberdeen Angus 0.722, and Hereford 0.683. Research data certified the possibility of using the Finnish Bovine Genotypes Panel 3.1 of DNA microsatellites markers for the cattle breed discrimination.
- The aim of this study was utilize microsatellites for routine parentage testing and identification in breed cattle discrimination. The analysis was performed in three beef cattle (Aberdeen Angus n= 93, Hereford n= 125, Charolais n = 101) and one dairy cattle breed (Czech Fleckvieh n = 46). In total, 365 animals were genotyped with using Finnish Bovine Genotypes Panel 3.1 for 18 microsatellite markers recommended by both ISAG for parentage testing and FAO for genetic studies of domestic animals (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824, BM1818, and SPS113, RM067, CSRM60, MGTG4B, CSSM66, ILSTS006, respectively). The STRUCTURE (version 2.2) program based on a Bayesian clustering method was used to obtain population structure. The software detected four clusters corresponding to the four breeds without using prior information. The genetic markers used in study were suitable for good clustering ability by evidencing good discrimination power. Differentiation among individuals of each breed characterized by Fst values range from the lowest 0.091 in Aberdeen Angus, 0.099 in Czech Fleckvieh, 0.122 in Charolais, to highest 0.146 in Hereford cattle population. Average distances (expected heterozygosity) between individuals in same cluster were for Czech Fleckvieh 0.684, Charolais 0.702, Aberdeen Angus 0.722, and Hereford 0.683. Research data certified the possibility of using the Finnish Bovine Genotypes Panel 3.1 of DNA microsatellites markers for the cattle breed discrimination. (en)
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