About: Magnetic particles-based biosensor for biogenic amines using an optical oxygen sensor as a transducer

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/article/10.1007%2Fs00604-012-0932-0
Description	We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercial microbeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic-organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25-30 mu mol L-1, and responses are linear up to a concentration of 1 mmol L-1. We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercial microbeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic-organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25-30 mu mol L-1, and responses are linear up to a concentration of 1 mmol L-1. (en)
Title	Magnetic particles-based biosensor for biogenic amines using an optical oxygen sensor as a transducer Magnetic particles-based biosensor for biogenic amines using an optical oxygen sensor as a transducer (en)
skos:prefLabel	Magnetic particles-based biosensor for biogenic amines using an optical oxygen sensor as a transducer Magnetic particles-based biosensor for biogenic amines using an optical oxygen sensor as a transducer (en)
skos:notation	RIV/61989592:15310/13:33147699!RIV14-MSM-15310___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED0007/01/01), P(IAAX08240901)
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Šafařík, Ivo Šebela, Marek Pospíšková, Kristýna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	85720
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/13:33147699
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ruthenium complex; Fluorescence quenching; Diamine oxidase; Optical fibre biosensor; Magnetic carriers (en)
http://linked.open.../riv/klicoveSlovo	Diamine oxidase Magnetic carriers Optical fibre biosensor Fluorescence quenching Ruthenium complex
http://linked.open...odStatuVydavatele	AT - Rakouská republika
http://linked.open...ontrolniKodProRIV	[7D87C2CFE0E4]
http://linked.open...i/riv/nazevZdroje	Microchimica Acta
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Novel inorganic-organic hybrid nanomaterials Centre of the Region Haná for Biotechnological and Agricultural Research
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	180
http://linked.open...iv/tvurceVysledku	Kuncová, Gabriela Šafařík, Ivo Šebela, Marek Pospíšková, Kristýna
http://linked.open...ain/vavai/riv/wos	000313837900017
issn	0026-3672
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00604-012-0932-0
http://localhost/t...ganizacniJednotka	15310

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