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rdf:type
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Description
| - Background: PTEN is a tumour suppressor protein with phosphatase activity frequently altered in several types of human cancers. Materials and Methods: The PTEN effect was studied on the cell cycle (by bromdeoxyuridine incorporation) and on the phosphatidylinositol-3-kinaselprotein kinase B/Akt (PI3-K/PKB/Akt) pathway regulating proteins (by immunocytochemical, Western blot analysis and kinase assay) upon transfection of wild-type PTEN and its mutant H123Y in breast cancer cell lines. Results: The expression of the important proteins in the MCF-7 and BT-549 cells was characterised and the cellular localisation of PTEN was analysed. Transfection of H123Y led to the down-regulation of p27(KiP1) and p21(Waf1/Cip1) protein levels and the up-regulation of phosphorylated PKB/Akt. An overexpression of PTEN decreased cyclin E/cdk2 activity and inhibited S-phase entry in MCF-7. In BT-549 these changes were not observed, but overexpression of PTEN led to a diminution of PKB/Akt phosphorylation. Conclusion: PTEN
- Background: PTEN is a tumour suppressor protein with phosphatase activity frequently altered in several types of human cancers. Materials and Methods: The PTEN effect was studied on the cell cycle (by bromdeoxyuridine incorporation) and on the phosphatidylinositol-3-kinaselprotein kinase B/Akt (PI3-K/PKB/Akt) pathway regulating proteins (by immunocytochemical, Western blot analysis and kinase assay) upon transfection of wild-type PTEN and its mutant H123Y in breast cancer cell lines. Results: The expression of the important proteins in the MCF-7 and BT-549 cells was characterised and the cellular localisation of PTEN was analysed. Transfection of H123Y led to the down-regulation of p27(KiP1) and p21(Waf1/Cip1) protein levels and the up-regulation of phosphorylated PKB/Akt. An overexpression of PTEN decreased cyclin E/cdk2 activity and inhibited S-phase entry in MCF-7. In BT-549 these changes were not observed, but overexpression of PTEN led to a diminution of PKB/Akt phosphorylation. Conclusion: PTEN (en)
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Title
| - Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells
- Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells (en)
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skos:prefLabel
| - Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells
- Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells (en)
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skos:notation
| - RIV/61989592:15310/06:00010655!RIV10-MSM-15310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA204/01/0488), P(NC6779), P(NR7828), Z(AV0Z50380511), Z(MSM6198959216)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/06:00010655
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - breast cancer cell lines; cell cycle; phosphatase activity; PKB/Akt; PTEN; tumour suppressor; p27(Kip1); p21(Waf1/Cip1); p53 and pRb (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Kryštof, Vladimír
- Kolář, Zdeněk
- Šváchová, Michaela
- Hlobilková, Alice
- Knillová, Jana
- Skypalová, Petra
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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